Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RiboTag and bulk RNA sequencing using AAV-shGpr151 and AAV-shGpr151 with AAV-e5'UTR infected sensory neurons.

ABSTRACT: After discovering that Gpr151 mRNA regulates the CSDE1-mediated rp-mRNA operon, we designed an engineered version of the 5'UTR of Gpr151 mRNA(e5'UTR), which has an enhanced interaction with CSDE1. To determine if this e5'UTR RNA can restore the down-regulated rp-mRNA operon formation in Gpr151 knockdowned neurons, we performed total RNA and RiboTag sequencing analysis using AAV-shGpr151 or AAV-shGpr151 with AAV-e5'UTR infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+.

ORGANISM(S): Mus musculus

PROVIDER: GSE301325 | GEO | 2025/09/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

DRG tissue CSDE1-RIP and bulk RNA sequencing using sciatic nerve injured and uninjured AAV-Control or AAV-shGpr151 injected mice.

Project description:To determine the effect of Gpr151 mRNA depletion to rp-mRNA operon including RNA-binding protein CSDE1 after injury, we conducted CSDE1-IP sequencing analysis using the dorsal root ganglions(DRGs) of AAV-control and AAV-shGpr151 injected mice.

2025-09-01 | GSE301322 | GEO

RiboTag and bulk RNA sequencing using AAV-Control and AAV-shGpr151 infected sensory neurons.

Project description:To investigate the role of the Gpr151 mRNA and its interaction with ribosomal protein mRNA operon in regenerating neurons, we performed total RNA and RiboTag sequencing analysis using AAV-Control or AAV-shGpr151 infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+.

2025-09-01 | GSE301324 | GEO

CSDE1-RIP and bulk RNA sequencing using primary DRG neurons infected with LV-control or LV-e5'UTR.

Project description:To determine overexpression of e5'UTR can enhance injury responsive rp-mRNA operon without injury, we conducted CSDE1-IP sequencing analysis using primary dorsal root ganglion (DRG) neurons infected with LV-control and LV-e5'UTR.

2025-09-01 | GSE301323 | GEO

Sciatic nerve injury-responsive differential CSDE1 association efficiency of ribosomal protein coding mRNAs

Project description:Gpr151 is an injury-responsive gene that promotes axon regeneration through its interaction with the 5'UTR region of its mRNA transcripts and the RNA-binding protein CSDE1. We found that knocking down or overexpressing the 5'UTR of Gpr151 changes the ribosomal protein mRNA regulon regulated by CSDE1. After the sciatic nerve injury, the association between the ribosomal protein mRNA regulon and CSDE1 was enhanced at dorsal root ganglions(DRGs), but this enhancement was not observed in the AAV-shGpr151 injected mice. Overexpression of 5'UTRmut, which has increased binding affinity to CSDE1, enhanced the ribosomal protein mRNA regulon and its association with CSDE1 in embryonic DRG neurons.

2023-08-18 | GSE236910 | GEO

RiboTag and bulk RNA sequencing using AAV-Control and AAV-shCsde1 infected sensory neurons.

Project description:Peripheral neurons can regenerate after nerve injury. To investigate the role of the RNA-binding protein CSDE1 and its interaction with ribosomal protein mRNAs in regenerating neurons, we performed total RNA and RiboTag sequencing analysis using AAV-Control or AAV-shCsde1 infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+.

2025-09-01 | GSE301320 | GEO

RiboTag and bulk RNA sequencing using AAV-Control and AAV-shLarp1 infected sensory neurons.

Project description:To investigate the role of the RNA-binding protein LARP1 and its interaction with ribosomal protein mRNAs in regenerating neurons, we performed total RNA and RiboTag sequencing analysis using AAV-Control or AAV-shLarp1 infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+.

2025-09-01 | GSE301327 | GEO

Promoting axon regeneration by enhancing the non-coding function of the injury-responsive coding gene Gpr151

Project description:Gene expression profiling in response to nerve injury has been mainly focused on protein functions of coding genes to understand mechanisms of axon regeneration and to identify targets of potential therapeutics for nerve repair. However, the protein functions of several highly injury-induced genes including Gpr151 for regulating the regenerative ability remain unclear. Here we present an alternative approach focused on non-coding functions of the coding genes, which led to the identification of the non-coding function of Gpr151 RNA interacting with RNA-binding proteins such as CSDE1. Gpr151 promotes axon regeneration by the function of its 5’- untranslated region (5’UTR) and expression of an engineered form of the 5’UTR improves regenerative capacity in vitro and in vivo in both sciatic nerve and optic nerve injury models. Our data suggest that searching injury-induced coding genes potentially functioning by their non-coding regions is required for the RNA-based gene therapy for improving axon regeneration.

2021-07-01 | GSE179145 | GEO

A multi-functional AAV-CRISPR-Cas9 and its host response

Project description:CRISPR-Cas9 delivery by AAV holds promise for gene therapy but faces critical barriers due to its potential immunogenicity and limited payload capacity. Here, we demonstrate genome engineering in postnatal mice using AAV-split-Cas9, a multi-functional platform customizable for genome-editing, transcriptional regulation, and other previously impracticable AAV-CRISPR-Cas9 applications. We identify crucial parameters that impact efficacy and clinical translation of our platform, including viral biodistribution, editing efficiencies in various organs, antigenicity, immunological reactions, and physiological outcomes. These results reveal that AAV-CRISPR-Cas9 evokes host responses with distinct cellular and molecular signatures, but unlike alternative delivery methods, does not induce detectable cellular damage in vivo. Our study provides a foundation for developing effective genome therapeutics mRNA-Seq from muscles (9 samples; 3 mice x 3 conditions) and lymph nodes (9 samples; 3 mice x 3 conditions).

2016-07-29 | E-GEOD-84534 | biostudies-arrayexpress

VLDL Proteome in Control-AAV and PSS1(Q353R)-AAV

Project description:Fractionated VLDL from Control-AAV and PSS1(Q353R)-AAV.

2024-10-21 | PXD047916 | Pride

miRNA and mRNA expression profiling of hepatocellular carcinoma induced by AAV in vivo gene targeting at the Rian locus

Project description:This SuperSeries is composed of the following subset Series: GSE35402: miRNA expression profiling of hepatocellular carcinoma induced by AAV in vivo gene targeting at the Rian locus GSE35403: mRNA expression profiling of hepatocellular carcinoma induced by AAV in vivo gene targeting at the Rian locus Refer to individual Series

2012-07-02 | E-GEOD-35404 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data