Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell level gene expression profiles of adult Drosophila midguts with lineage tracing targeting enteroendocrine cells

ABSTRACT: Differentiated cells exhibit cellular plasticity,where they dedifferentiate into stem cells. We studied cellular plasticity of secretory enternendocrine cells (EEs) in the fruit fly Drosophila melanogaster. We used flies that had EEs lineage-traced and resolved single-cell transcriptomics of all cell types in their midguts. Our scRNA-seq data have characterized cell-type-gene expression profiles, with a specific focus on EE lineages.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE301510 | GEO | 2025/07/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell level gene expression profiles of enteroendocrine cell lineages, control guts and guts with Xrp1 overexpressed in EEs

Project description:Differentiated cells exhibit cell plasticity, where they dedifferentiate to stem cells or trans-differentiate into cells of another lineage, when necessary. We studied the cell plasticity of secretory enternendocrine cells (EEs) in the fruit fly Drosophila melanogaster. We resolved single-cell transcriptomics of EEs and cells derived from EEs. Our scRNA-seq data of EE lineages have characterized intermediate cell types derived from EEs. We also specifically focused on a gene called Xrp1 and examined potential changes of all epithelial cell types by EE-specific Xrp1 overexpression (O/E). Our scRNA-seq data of Xrp1 O/E EEs have provided evidence on changes in gene expression profiles of each cell type by Xrp1 O/E in EEs.

2025-07-07 | GSE295684 | GEO

A hierarchical transcription factor cascade regulates enteroendocrine cell diversity and plasticity in Drosophila [DamID-Seq]

Project description:Enteroendocrine cells (EEs) represent a heterogeneous cell population in intestine and exert endocrine functions by secreting a diverse array of neuropeptides. Although many transcription factors (TFs) required for specification of EEs have been identified in both mammals and Drosophila, it is not understood how these TFs work together to generate this considerable subtype diversity. Here we show that EE diversity in adult Drosophila is generated via an “additive hierarchical TF cascade”. Specifically, a combination of a master TF, a secondary-level TF and a tertiary-level TF constitute a “TF code” for generating EE diversity. We also discover a high degree of post-specification plasticity of EEs, as changes in the code—including as few as one distinct TF—allow efficient switching of subtype identities. Our study thus reveals a hierarchically-organized TF code that underlies EE diversity and plasticity in Drosophila, which can guide investigations of EEs in mammals and inform their application in medicine.

2022-10-18 | GSE211629 | GEO

A hierarchical transcription factor cascade regulates enteroendocrine cell diversity and plasticity in Drosophila [RNA-Seq]

2022-10-18 | GSE211628 | GEO

Single-cell sequencing of enteroendocrine cells (EEs) in Drosophila midgut.

Project description:Enteroendocrine cells (EEs) in the intestinal epithelium have important endocrine functions, yet this cell lineage exhibits great local and regional variations that have hampered detailed characterization of EE subtypes. Through single cell RNA-sequencing analysis, combined with a collection of neuropeptide/receptor knock-in strains, here we provide a comprehensive analysis of cellular diversity, spatial distribution and transcription factor (TF) code of EEs in adult Drosophila midgut. We identify 10 major EE subtypes that totally produced 14 different classes of hormone peptides. Each EE on average co-produces 2-5 different classes of hormone peptides. Functional screen with subtype-enriched TFs suggests a combinatorial TF code that controls EE cell diversity, with class-specific TFs Mirr and Ptx1that respectively define two major classes of EEs, and regional TFs such as Esg, Drm, Exex and Fer1that further define regional EE identity. Our single-cell data should greatly facilitate Drosophila modeling of EE differentiation and function.

2019-11-04 | GSE132274 | GEO

Efficient trans-differentiation/ de-differentiation of mature intestinal cells in adult Drosophila midgut by depleting a single transcription factor

Project description:The manipulation of cell identity by reprograming holds immense potential in regenerative medicine, but is often limited by the inefficient acquisition of fully functional cells. This problem can potentially be resolved by better understanding the reprogramming process using in vivo genetic models, which are currently scarce. Here we report that both enterocytes (ECs) and enteroendocrine cells (EEs) in adult Drosophila midgut show a surprising degree of cell plasticity. Depleting the transcription factor Tramtrack in the differentiated ECs can initiate Prospero-mediated cell transdifferentiation leading to EE-like cells. On the other hand, depletion of Prospero in the differentiated EEs leads to the loss of EE-specific transcription programs, allowing cell cycle re-entry or differentiation into ECs. We found that intestinal progenitor cells, ECs, and EEs have a similar chromatin accessibility profile, suggesting that cell plasticity is enabled by pre-existing chromatin accessibility with switchable transcription programs. In a pilot genetic screen using this system, we found that cell lineage confliction and epigenetic memory were potential barriers during the EC-to-EE transdifferentiation. The establishment of this genetically tractable in vivo model should facilitate mechanistic investigation of cell plasticity at the molecular and genetic level.

2024-03-04 | GSE235505 | GEO

RNA-seq analysis of young vs. old Drosophila melanogaster posterior midguts

Project description:The goal of this study is to compare changes in gene expression between young (5 days old) and old (45 days old) flies posterior midguts.

2016-12-18 | GSE74171 | GEO

Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fatbodies

Project description:Survey of relative gene expression profiles in larvae, pupae, and midguts and fatbodies of teneral, unfed, and fed adult mountain pine beetles from western Nevada, USA.

2009-08-29 | GSE17858 | GEO

RNA-seq analysis of Drosophila melanogaster posterior midguts

Project description:RNA-seq analysis of Drosophila melanogaster posterior midguts

| PRJNA299213 | ENA

Robust differentiation of human enteroendocrine cells from intestinal stem cells

Project description:Enteroendocrine (EE) cells are the most abundant hormone-producing cells in humans and are critical regulators of energy homeostasis and gastrointestinal function. Challenges in converting human intestinal stem cells (ISCs) into functional EE cells, ex vivo, have limited progress in elucidating their role in disease pathogenesis and in harnessing their therapeutic potential. To address this, we employed small molecule targeting of key transcriptional regulators, GATA4, JNK and FOXO1, known to mediate endodermal development and hormone production, together with directed differentiation of human ISCs from the duodenum and rectum. We observed marked induction of EE cell differentiation and gut-derived expression and secretion of SST, 5HT, GIP, GLP-1 and PYY upon treatment with various combinations of three small molecules: rimonabant, SP600125 and AS1842856. Robust differentiation strategies capable of driving human EE cell differentiation is a critical step towards understanding these essential cells and the development of cell-based therapeutics.

2021-11-17 | GSE178342 | GEO

Relative quantitative proteomics of uninfected vs wMel infected Aedes aegypti midguts

Project description:Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important new dengue control strategy. To better understand the mechanisms of virus inhibition, proteomic quantification of the effects of Wolbachia in mosquito (Aedes aegypti) midguts was performed.

2017-09-19 | PXD006239 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data