Application of Microdroplet PCR for Large-Scale Targeted Bisulfite Sequencing

ABSTRACT: Cytosine methylation of DNA CpG dinucleotides in gene promoters is an epigenetic modification that regulates gene transcription. While many methods exist to interrogate methylation states, no current methods offer large-scale, targeted, single CpG resolution. We report an approach combining bisulfite treatment followed by RainDance microdroplet PCR with next-generation sequencing to assay the methylation state of 50 genes in the regions 1 kb upstream and downstream of their transcription start sites.

ORGANISM(S): Homo sapiens

PROVIDER: GSE30179 | GEO | 2011/06/25

SECONDARY ACCESSION(S): PRJNA143939

REPOSITORIES: GEO