Genome-wide chromatin accessibility profiling of Prdm12-edited CD8+ T cells by ATAC-seq

ABSTRACT: This study investigates the impact of Prdm12 knockout on chromatin accessibility in CD8+ T cells. CD8+ T cells were isolated from spleens of OTI:Cas9 transgenic mice (C57BL/6 background) using magnetic bead-based negative selection. Isolated cells were subjected to CRISPR-Cas9 mediated gene editing targeting Prdm12 (Prdm12-KO group) or a non-targeting control guide RNA (Control group) via ribonucleoprotein (RNP) complex electroporation. ATAC-seq was performed to compare genome-wide chromatin accessibility between edited (treatment) and non-edited (control) cells, aiming to identify regulatory regions associated with Prdm12 function.

ORGANISM(S): Mus musculus

PROVIDER: GSE301907 | GEO | 2025/08/20

REPOSITORIES: GEO