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ScRNA-Seq of Retinal microglia from 6 months old Balb/c mice infected with MCMV at birth

ABSTRACT: We used a single cell sequencing approach using 10x Genomics scRNAseq to understand the transcriptional differences between microglia from MCMV infected animals vs controls. MCMV infection at birth resulted in increased retinal degeneration with age. Microglia from mice infected with MCMV appeared to have an alternative activation state compared to age matched uninfected controls. We utilized a depletion and repopulation strategy to reset the population of microlgia using PLX5622, a CSFR1 inhibitor. This study allowed analysis of the transcriptional changes caused by MCMV infection early in life potentially contributing to neurodegeneration.

ORGANISM(S): Mus musculus

PROVIDER: GSE302157 | GEO | 2026/01/29

REPOSITORIES: GEO

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