Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional control of meiotic recombination by the DREAM complex [CUT&TAG]

ABSTRACT: The meiotic processes of chromosome segregation and recombination, crucial for sexual reproduction and evolution, require a specialized gene set. However, the mechanisms governing the expression of these genes remain largely elusive in multicellular eukaryotes. Here, we identify the DREAM complex as a transcriptional regulator of meiotic recombination in Arabidopsis. We find that the DREAM complex localizes to meiotic chromosomes and reducing DREAM function leads to defects in meiotic recombination causing sterility. Meiocyte-specific transcriptomics together with genome-wide analysis of DREAM-bound genes revealed recombination-associated genes, including the crossover-promoting helicase MER3, as direct targets of the DREAM complex. Bypassing DREAM regulation of MER3 partially restored recombination defects in plants with reduced DREAM activity. Our data unravel the DREAM complex as a major regulator of meiosis. Given that reduction of the DREAM function is associated with reproductive defects in other species, such as Drosophila and mice, this function might be conserved among multicellular eukaryotes.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE302286 | GEO | 2026/09/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional control of meiotic recombination by the DREAM complex [RNA-Seq]

Project description:The meiotic processes of chromosome segregation and recombination, crucial for sexual reproduction and evolution, require a specialized gene set. However, the mechanisms governing the expression of these genes remain largely elusive in multicellular eukaryotes. Here, we identify the DREAM complex as a transcriptional regulator of meiotic recombination in Arabidopsis. We find that the DREAM complex localizes to meiotic chromosomes and reducing DREAM function leads to defects in meiotic recombination causing sterility. Meiocyte-specific transcriptomics together with genome-wide analysis of DREAM-bound genes revealed recombination-associated genes, including the crossover-promoting helicase MER3, as direct targets of the DREAM complex. Bypassing DREAM regulation of MER3 partially restored recombination defects in plants with reduced DREAM activity. Our data unravel the DREAM complex as a major regulator of meiosis. Given that reduction of the DREAM function is associated with reproductive defects in other species, such as Drosophila and mice, this function might be conserved among multicellular eukaryotes.

2026-09-09 | GSE302287 | GEO

The Pif1 helicase is actively inhibited during meiotic recombination to prevent excess length of gene conversions

Project description:Meiotic recombination ensures proper chromosome segregation to form viable gametes and results in gene conversion events between homologs. Conversion tracts are shorter in meiosis than in mitotically dividing cells. This results at least in part from the binding of a complex, formed of the Mer3 helicase and the MutL heterodimer, to meiotic recombination intermediates. The molecular actors inhibited by this complex are elusive. The Pif1 DNA helicase is known to stimulate DNA polymerase delta (Pol δ) -mediated DNA synthesis from D-loops in vitro, allowing long synthesis required for break-induced replication. We show that Pif1 is recruited genome wide to meiotic double-strand break (DSB) sites. We further show that Pif1, through its interaction with PCNA, is required for the long gene conversions observed in the absence of MutL recruitment to recombination sites. in vivo, Mer3 interacts with the PCNA clamp loader RFC, and in vitro, Mer3-MutL ensemble inhibits Pif1-stimulated D-loop extension by Pol δ and RFC-PCNA. Mechanistically, our results suggest that Mer3-MutL may compete with Pif1 for binding to RFC-PCNA. Taken together, our data show that Pif1’s activity to promote meiotic recombination-related DNA synthesis is restrained by the Mer3-MutL ensemble to prevent long gene conversion events and possibly associated mutagenesis.

2021-03-01 | GSE164467 | GEO

Transcriptional control of meiotic recombination by the DREAM complex [RNA-Seq]

Project description:Transcriptional control of meiotic recombination by the DREAM complex [RNA-Seq]

| PRJNA1289802 | ENA

Transcriptional control of meiotic recombination by the DREAM complex [CUT&TAG]

Project description:Transcriptional control of meiotic recombination by the DREAM complex [CUT&TAG]

| PRJNA1289809 | ENA

Biochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates

Project description:Crossing over between homologs is critical for the stable segregation of chromosomes during the first meiotic division. S. cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of the majority of crossovers during meiosis. Here we describe the structural organisation of Mer3 and using AlphaFold modelling and XL-MS we further characterise the previously described interaction with Mlh1-Mlh2. We find that Mer3 also forms a previously undescribed complex with the recombination regulating factors Top3 and Rmi1 and that this interaction is competitive with Sgs1BLM helicase in a phospho-dependent manner. Using in vitro reconstituted D-loop assays we show that Mer3 inhibits the anti-recombination activity of Sgs1 helicase, but only in the presence of Dmc1. Thus we provide a mechanism whereby Mer3 interacts with a network of proteins to protect Dmc1 derived D-loops from dissolution.

2023-07-20 | PXD039839 | Pride

RPA directly stimulates Mer3/HFM1 helicase processivity to ensure normal crossover formation in meiosis

Project description:Meiotic crossover formation is critical for generating viable gametes and enhancing genetic diversity. The helicase Mer3 (HFM1 in humans) is a highly conserved factor essential for promoting crossovers and ensuring their proper distribution. Here, we identify replication protein A (RPA) as a direct interactor of budding yeast Mer3. We demonstrate that this interaction is conserved between human HFM1 and RPA. Cross-linking mass spectrometry and structural modelling with AlphaFold2 reveal a conserved and specific Mer3-RPA interface. Single-molecule magnetic tweezers assays demonstrate that direct RPA interaction is required for Mer3 helicase processivity under conditions of low DNA tension. Consistently, mer3 mutant deficient in RPA binding exhibit reduced crossover frequencies and accumulate unresolved recombination intermediates during budding yeast meiosis. Via genome-wide localisation experiments, we link this effect to a weakened recruitment to double-strand break sites of the mer3 mutant. Our findings provide mechanistic insights into coordination of meiotic recombination by the Mer3 helicase through interactions with the canonical DNA repair machinery, highlighting a conserved mechanism underlying crossover control during sexual reproduction.

2025-11-02 | GSE305755 | GEO

The DREAM complex antagonizes WDR5a and represses the productive elongation of transcription in Arabidopsis [RNA-seq]

Project description:The master transcriptional repressor DREAM (dimerization partner, RB-like, E2F and multi-vulval class B) complex regulates cell cycle in eukaryotes, but much remains unknown about how it transmits repressive signals on chromatin to the primary transcriptional machinery (e.g., Pol II). Through a genetic screen, we identified BTE1 (barrier of transcription elongation 1), a key component in the plant DREAM complex. The subsequent characterization demonstrated that the DREAM complex represses Pol II elongation. The core DREAM subunits E2F transcription factors recruit BTE1 to accumulate at the promoter-proximal regions of target genes. DREAM target genes exhibit characteristic enrichment of H2A.Z and H3K4me2 modification on chromatin. The DREAM complex BTE1 subunit interacts with WDR5a, repressing WDR5a chromatin binding and the productive elongation of transcription on BTE1-targeted genomic loci. Thus, we demonstrate a major role of DREAM complex in the control of transition from transcription initiation to productive elongation around TSSs on the open chromatin via inhibiting WDR5a function.

2022-06-28 | GSE181484 | GEO

The DREAM complex antagonizes WDR5a and represses the productive elongation of transcription in Arabidopsis [ChIP-seq]

2022-06-28 | GSE181486 | GEO

The DREAM complex antagonizes WDR5a and represses the productive elongation of transcription in Arabidopsis [GRO-seq]

2022-06-28 | GSE181488 | GEO

The DREAM complex functions as conserved master regulator of somatic DNA repair capacities

Project description:Mutations in the DREAM complex induce germline-like gene expression patterns including the increase of multiple mechanisms of DNA repair in the soma of Caenorhabditis elegans. This confers mutants of the DREAM complex resistance to a wide range of DNA damage types during development and aging.

2023-01-31 | PXD033836 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data