Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Evaluation of KDAC6 (HDAC6) inhibitor selectivity in HT1080 cells

ABSTRACT: To investigate the specificity and biological impacts of KDAC-selective inhibitors, we treated wild-type HT1080 cells with KDAC6 selective inhibitors SW-113 and SE-138. Expression profiling analysis was then performed on the treated cells vs. untreated wild-type HT1080 cells. Comparable wild-type data is available in GSE302260.

ORGANISM(S): Homo sapiens

PROVIDER: GSE302384 | GEO | 2026/07/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Evaluation of genetic inactivation of lysine deacetylases KDAC6 (HDAC6) and KDAC8 (HDAC8) versus treatment with KDAC-selective inhibitors

Project description:To investigate the specificity and biological impacts of KDAC-selective inhibitors, we treated wild-type HT1080 cells and cell lines containing point mutations that result in endogenous expression of a catalytically-inactive variant with KDAC6 and KDAC8 selective inhibitors Tubastatin A and PCI-34051. Expression profiling analysis was then performed on the treated cells vs. untreated wild HT1080 cells.

2024-02-15 | GSE251743 | GEO

Endogenous expression of inactive lysine deacetylases KDAC6 (HDAC6) and KDAC8 (HDAC8)

Project description:To investigate the roles of KDAC6 and KDAC8, we generated cell lines containing point mutations in HT1080 cells that result in endogenous expression of a catalytically-inactive variant. Expression profiling analysis was then performed on the resulting cell lines vs. wild HT1080 cells and cells treated with KDAC inhibitors tubastatin A or PCI-34051.

2023-09-08 | GSE228549 | GEO

Gene expression effects of KDAC6 (HDAC6) knockout in HT1080 cells

Project description:To study the gene expression and phenotype roles of KDAC6, CRISPR/Cas9 was used to create knockout cell lines from wild-type HT1080 cells. Four individual isolated knockout clones were pooled to create the line. Comparable wild-type data is available in GSE302260.

2025-08-10 | GSE302329 | GEO

Endogenous expression of inactive lysine deacetylases KDAC4 (HDAC4) and KDAC7 (HDAC7)

Project description:To investigate the roles of KDAC4 and KDAC7, we generated cell lines containing point mutations in HT1080 cells that result in endogenous expression of mutated proteins. For KDAC4, we introduced a mutation (H803A) to inactivate catalysis but that also contains concurrent mutations resulting in a knockout, as well as one that enhances in vitro activity of KDAC4 (H976Y, heterozygous with a knockout mutation on the second chromosal copy). For KDAC7, an inactivating mutation (H709A, homozygous) was introduced. Expression profiling analysis was then performed on the resulting cell lines and wild-type HT1080 cells treated with the KDAC inhibitor TMP195, vs. untreated wild-type HT1080.

2026-07-01 | GSE250542 | GEO

RNA-seq of wild-type HT1080 fibrosarcoma cells

Project description:RNA-seq of wild-type HT1080 fibrosarcoma cells

| PRJNA1289741 | ENA

Gene expression effects of KDAC8 (HDAC8) knockout in HT1080 cells

Project description:To study the gene expression and phenotype roles of KDAC8, CRISPR/Cas9 was used to create knockout cell lines from wild-type HT1080 cells. Four individual isolated knockout clones were pooled to create the line. Comparable wild-type data is available in GSE302260.

2025-08-10 | GSE302330 | GEO

Gene expression profile changes in HDAC6 knockdown HT1080 cells cells after TLR3 stimulation

Project description:TLR3 stimulation by extracellular dsRNA (e.g. polyIC) induces expression of numerous genes. The knockdown of HDAC6 prior to TLR3 stimulation leads to an ablation of expression of IRF-3-dependent genes. Total RNA extracted from HT1080 cells expressing control-shRNA or HDAC6-shRNA, after 6 h of treatment with dsRNA (polyIC), compared to untreated controls

2013-12-31 | E-GEOD-43218 | biostudies-arrayexpress

Gene expression effects of KDAC6 (HDAC6) knockout in HT1080 cells

Project description:Gene expression effects of KDAC6 (HDAC6) knockout in HT1080 cells

| PRJNA1290097 | ENA

Evaluation of genetic inactivation of lysine deacetylases KDAC6 (HDAC6) and KDAC8 (HDAC8) versus treatment with KDAC-selective inhibitors

Project description:Evaluation of genetic inactivation of lysine deacetylases KDAC6 (HDAC6) and KDAC8 (HDAC8) versus treatment with KDAC-selective inhibitors

| PRJNA1055155 | ENA

Expression data from RECK-overexpressing HT1080 fibrosarcoma cells

Project description:RECK, a glycosylphosphatidylinositol-anchored glycoprotein, inhibits the enzymatic activities of some matrix metalloproteinases (MMP), thereby suppressing tumor cell metastasis; however, the detailed mechanism is still obscure. In this study, we compared the gene expression profiles between mock- and RECK-transfected HT1080 cells. Three established cell lines were selected for RNA extraction and hybridization on Affymetrix microarrays: (1) mock-transfected HT1080 cells, designated as HT1080-Zeo, (2) RECK-overexpressing HT1080 cells, designated as HT1080-RECK, and (3) RECK/4NQ-overexpressing HT1080 cells (in which four N-glycosylation asparagine residues of RECK (Asn86, Asn200, Asn297, and Asn352) were replaced with glutamine residues), designated as HT1080-RECK/4NQ.

2010-04-14 | E-GEOD-21306 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data