Inflammatory memory primes the intestine for enhanced regenerative potential [multiome 2]

ABSTRACT: Intestinal regeneration after injury can occur through cellular plasticity, whereby differentiated epithelial cells dedifferentiate to replenish the intestinal stem cell (ISC) pool. To investigate whether this dedifferentiation process leads to lasting transcriptomic and epigenetic changes, we used Krt20CreERT2:Rosa26-tdTomato mice to specifically label villus epithelial cells. In non-irradiated conditions, these cells are rapidly shed and lost. However, following irradiation, a subset of labeled cells dedifferentiates, migrates into the crypt compartment, and contributes to ISC regeneration. To characterize long-term molecular changes in these dedifferentiated cells and their progeny, we performed single-cell multiome sequencing (scRNA-seq and scATAC-seq) using the 10x Genomics Chromium platform.

ORGANISM(S): Mus musculus

PROVIDER: GSE302687 | GEO | 2026/09/16

REPOSITORIES: GEO