Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Rapid maize leaf and immature ear responses to UV-B radiation

ABSTRACT: This study looked for early response signals produced in UV-B irradiated leaves and induced in shielded leaves and shielded ears that modulate physiological responses in maize. Transcriptome profiling tracked changes in exposed and shielded organs. Metabolic profiling was examined for signaling molecules. The top 2 leaves were exposed to 10, 30, or 60 minutes of ~3x normal UV-B and then tissue samples were taken from all 3 tissue types. Over 250 transcripts are differentially expressed at least 2 fold in irradiated leaves by 10 min of UV-B. At 30 min this decreased to ~150 then increased to over 500 transcripts at 60 min. Different pathways are affected at the various time exposures and specific responses are modulated by the time of exposure. Shielded leaves had a smaller number of transcripts affected at each time point but over 60% were in common with the irradiated leaves results at 10 min. and ~20% overlaps were found at 30 and 60 min. Metabolite samples were analyzed after 5, 10, 15, 30, and 90 min of UV-B. We identified 14 compounds with a statistically significant change from untreated plants in at least one time point.

ORGANISM(S): Zea mays

PROVIDER: GSE30278 | GEO | 2011/07/31

SECONDARY ACCESSION(S): PRJNA143601

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Rapid maize leaf and immature ear responses to UV-B radiation

Project description:This study looked for early response signals produced in UV-B irradiated leaves and induced in shielded leaves and shielded ears that modulate physiological responses in maize. Transcriptome profiling tracked changes in exposed and shielded organs. Metabolic profiling was examined for signaling molecules. The top 2 leaves were exposed to 10, 30, or 60 minutes of ~3x normal UV-B and then tissue samples were taken from all 3 tissue types. Over 250 transcripts are differentially expressed at least 2 fold in irradiated leaves by 10 min of UV-B. At 30 min this decreased to ~150 then increased to over 500 transcripts at 60 min. Different pathways are affected at the various time exposures and specific responses are modulated by the time of exposure. Shielded leaves had a smaller number of transcripts affected at each time point but over 60% were in common with the irradiated leaves results at 10 min. and ~20% overlaps were found at 30 and 60 min. Metabolite samples were analyzed after 5, 10, 15, 30, and 90 min of UV-B. We identified 14 compounds with a statistically significant change from untreated plants in at least one time point. Compared transciptomes of irradiated leaves, shielded leaves, and shielded ears from plants whose top 2 leaves were exposed to ~3x normal UV-B for 10, 30, and 60 minutes.

2011-07-30 | E-GEOD-30278 | biostudies-arrayexpress

Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize

Project description:This study looked for signals produced in UV-B irradiated leaves, and possibly induced in shielded leaves, that modulate physiological responses in maize. Transcriptome and proteomics profiling tracked changes in exposed and shielded organs. Metabolic profiling was examined for signaling molecules. Exposure of just the top leaf substantially alters the transcriptome of both irradiated and shielded organs, with greater changes as an additional 1-2 leaves are irradiated. Transcriptome, proteome and metabolome changes are UV-B regulated in shielded organs. Early steps in signal transduction and possible signal molecules are identified utilizing a time course experiment. Keywords: UVB, maize, leaves, ears Compared irradiated leaves in a time course, irradiated leaves versus shielded leaves, and shielded ears on plants with irradiated leaves. Also compared different number of leaves being irradiated. Spike-in controls were included.

2011-02-01 | E-GEOD-25038 | biostudies-arrayexpress

Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize

2011-02-01 | GSE25038 | GEO

Rapid molecular responses of maize to UV-B: gene expression profiling in irradiated and shielded tissues

Project description:Microarray hybridization was used to assess UV-B responses in both directly exposed and shielded tissues of a maize line deficient in anthocyanin accumulation. Among 347 transcripts found to be regulated after 8 h of high UV-B irradiation, 285 were increased significantly in at least one organ but only 80 were down-regulated by UV-B. Although some genes were regulated by UV-B in more than one sample, most UV-B regulated genes were organ-specific. A larger number of transcript changes occurred in directly exposed than in shielded organs, and more transcripts levels were changed in adult than in seedling tissues. Because shielded tissues including roots, immature ears, and leaves covered with a plastic that absorbs UV-B exhibited altered transcriptome profiles after plant exposure to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. The time course of transcript abundance changes monitored by RNA blot hybridization and real time RT-PCR indicated that the response kinetics to UV-B are very rapid as some transcript levels are altered within 1 h exposure, in both exposed and shielded tissues. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, transcriptome changes do not show reciprocity. Confirming and extending prior microarray hybridization results, supplemental UV-B increased expression of stress response and ribosomal protein genes, while photosynthesis-associated genes were down regulated in adult leaves. Keywords: other

2003-10-29 | GSE671 | GEO

Sugarcane transcriptome - Response to herbivory by Diatraea saccharalis

Project description:Sugarcane stalk borer larvae were grown on artificial diet and maintained at 25°C and 60±10% relative humidity with a 14 h/10 h light/dark cycle. Second instar larvae were maintained under fasting conditions for 18 h and transferred to two-month old plants (genotype SP80-3280, CTC, Brazil). Leaves were collected after 30 min and 24 h of exposure to herbivory for the control and experimental groups. Two plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

2007-05-20 | GSE4967 | GEO

Pea seedlings under UV-B irradiation

Project description:To investigate the mechanistic effects of UV irradiation on pea seedlings at the protein level, proteomic analyses were performed on pea seedlings irradiated daily with 0 and 10 min UV-B.

2023-05-04 | PXD038486 | Pride

Escherichia coli K-12: 5% isooctane treatment at four time points

Project description:Transcripitonal profiling of Escherichia coli K-12 BW25113 comparing cells with isooctane treatment at time point of 0, 10, 30 and 60 min with two biological replicates

2014-09-13 | GSE32131 | GEO

S. cerevisiae proteome response to heat stress - 240 min time course. C4PR_LIV

Project description:In this project, we follow the temporal proteome dynamics during heat stress response in a model organism Saccharomyces cerevisiae. Using label-free quantification we determine changes in the yeast proteome at different times after mild temperature shift, from 30˚C to 37˚C. The time points measured were 0 min (30˚C, no stress) and then at 10 min, 30 min, 60 min, 120 min and 240 min after cells were transferred to 37 °C. For each time point, four biological replicates were collected for the haploid wild type (BY4742, Mat ALPHA, his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; YJL088w::kanMX4) strain and a haploid strain with a chaperone Ssb1p deletion (ssb1Δ).

2018-01-17 | PXD006262 | Pride

Escherichia coli K-12: 5% isooctane treatment at four time points

Project description:Transcripitonal profiling of Escherichia coli K-12 BW25113 comparing cells with isooctane treatment at time point of 0, 10, 30 and 60 min with two biological replicates One-condition experiment, cells with isooctane treatment at incubation time of 0, 10, 30 and 60 min, respectively

2014-09-13 | E-GEOD-32131 | biostudies-arrayexpress

Identification of entertoxigenic Escherichia coli genes transcriptionally altered with the interaction of host cells

Project description:Genes identified in ETEC E24377A after interaction with host cells (Caco-2) after time points of 30 min, 60 min and 120 min. Both the adherent and non-adherent cells were profiled as well as E24377a grown only in tissue culture media.

2012-11-26 | GSE40427 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data