Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Radiofrequency regulates the BET-mediated pathways in radial glia differentiation in human cortical development

ABSTRACT: The human brain represents one of the most complex organs in our body, with development regulated by an intricate genetic program. Recently, non-genetic factors, such as prenatal stress, infection, and diet, have been shown to influence brain development. Radiofrequency radiation (RF; 800-2,400 MHz), emitted by natural and artificial sources such as microwaves and cell phones, represents a non-invasive environmental factor. Using human cortical organoids (hCOs) derived from human embryonic stem cells (hESCs), we investigate RF's effects on corticogenesis. We find that RF exposure regulates the differentiation of human and non-human primate radial glia progenitors, maintaining stem cell identity and delaying differentiation. Neurons differentiated under RF treatment show induction of expression of human endogenous retroviruses. Importantly, inhibitors for the BET (bromodomain and extraterminal) protein rescue RF-induced developmental defects in hCOs. Our findings reveal a mechanism by which RF modulates early brain development, offering a non-biological approach to regulate neural stem cell self-renewal.

ORGANISM(S): Homo sapiens

PROVIDER: GSE302899 | GEO | 2026/01/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Radiofrequency radiation impairs the human cortical organoid development

Project description:Neurodevelopmental disorders pose a severe health burden in our society, with remarkably increasing prevalence in children. Though radiofrequency radiation (RF) exposure to the prenatal brain is one of the environmental risks strongly associated with neurobehavioral disorders, its direct impact on human brain development remains largely unknown. Here we use human brain organoids representing the cortex, namely cortical organoids (hCOs) from human embryonic stem cells (hESC), to examine the effects of RF from mobile phones on corticogenesis. We found that RF exposure to brain organoids leads to structural impairment and the defective mitotic behaviors of radial glia progenitors, which carry out critical roles in neocortical expansion. We further uncovered that RF-treated organoids acquire the dysregulation in the transcriptional program, including the aberrant expression of autism risk genes and the morphologically and functionally abnormal neurons. Moreover, the RF shield made complete protection of the detrimental impact of RF on corticogenesis. Finally, we identified small molecule inhibitors for BET proteins that ameliorated RF-induced damages in cortical organoids. Our findings demonstrate how RF exposure impairs brain development and suggest new strategies to prevent RF-mediated brain damage.

2025-10-24 | GSE216700 | GEO

Neurodevelopmental cellular changes in rodents after 900 MHz radiofrequency exposureNeurodevelopmental cellular changes in rodents after 900 MHz radiofrequency exposure

Project description:This study investigates the effects of non-thermal levels of 900 MHz radiofrequency electromagnetic field (RF-EMF) which is used in mobile phone technology on neurodevelopment and neural stem cell differentiation. In vivo in the rat, the effects of pre- and post-natal 0.08 and 0.4 W/kg exposure were assessed on the proteomic profile at PND postnatal day 0 (PND) and on proliferation, synaptogenesis, and oxidative stress in the hippocampus and primary motor cortex of rats at PND 8 and PND 17. Complementary Mechanistic endpoints on cell differentiation were studied in vitro using stem cells exposed to 900 MHz RF-EMF at a non-thermal level for 3 or 7 days in vitro at the lowest SAR (0.08 W/kg). Our in vivo results showed a decrease in BDNF level and cells proliferation with a decrease in synapses balance. Increased proliferation, apoptosis, and double-strand DNA breaks in neural stem cells (NSCs) were also observed. Smaller ratio of B1 cells and a higher ratio of oligodendrocyte progenitor cells and astrocytes were observed in the exposed NSPCs. These findings suggest that developmental RF-EMF exposure induce changes in neurodevelopment in vivo from PND 0 and until PND 17 specifically on cellular proliferation supported by alterations in NSPCs in vitro, leading to the hypothesis of a vulnerability of developing central nervous system towards RF-EMF exposures at regulatory thresholds.

2025-09-11 | PXD053803 | Pride

Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene expression in MCF-7 cells

2010-07-20 | GSE22991 | GEO

Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene expression in MCF-7 cells

Project description:Despite many studies over a decade, it still remains ambiguous as to the real biological effects induced by radiofrequency electromagnetic fields (RF EMF). Epidemiological studies indicates that long-term exposure to EMF could increase the risk of breast cancer. Some reports have showed that in vitro EMF exposures change cellular gene expression. In this study, we investigated global gene expression responses to RF EMF simulating the Global System for Mobile Communications (GSM) 1800 MHz signal in human breast cancer cell line MCF-7 using transcriptomic approaches. MCF-7 cells were intermittently (5 min fields on/10 min fields off) exposed to RF EMF at an average specific absorption rate (SAR) of 2.0 W/kg (2 W/kg exposure group) or sham-exposed (2 W/kg control group) for 24 h, or exposed to RF EMF at a higher level of 3.5 W/kg (3.5 W/kg exposure group) or sham-exposed (3.5 W/kg control group).

2010-07-20 | E-GEOD-22991 | biostudies-arrayexpress

Proteomic profile of NE-4C cells after radiofrequency elecromagnetic field exposure and sham-exposure

Project description:NE-4C cells were randomly devided into sham group and RF exposure group, after 48 h 1950 MHz RF exposure at a SAR of 2W/kg, cells were harvested and proteomic profile was detected by liquid chromatography-tandem mass spectrometric approach.

2022-01-26 | PXD031285 |

2.45 GHz Radiofrequency fields alter gene expression in cultured human cells

Project description:The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF fields can cause changes in gene expression. We observed that 221 genes altered their expression after a 2-hour exposure. The number of affected genes increased to 759 after a 6-hour exposure. Functional classification of the affected genes reveals that apoptosis-related genes were among the up-regulated ones and the cell cycle genes among the down-regulated ones. We observed no significant increase in the expression of heat shock genes. These results indicate that the RF fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism. Keywords: gene expression SAGE We used the pulsed RF fields at a frequency of 2.45 GHz that is commonly used in telecommunication to expose cultured human HL-60 cells. We used the SAGE (serial analysis of gene expression) method to measure the RF effect on gene expression at the genome level.

2005-07-30 | E-GEOD-3025 | biostudies-arrayexpress

2.45 GHz Radiofrequency fields alter gene expression in cultured human cells

2005-07-30 | GSE3025 | GEO

Development of human brain organoids with functional vascular-like system

Project description:Human cortical organoids (hCOs), derived from human embryonic stem cells (hESCs), provide an excellent platform to study human brain development and diseases in complex 3D tissue. However, current hCOs lack microvasculature, resulting in limited oxygen and nutrient delivery to inner-most parts of hCOs. Previous studies demonstrated that the expression of human ETS variant 2 (hETV2) directly converts human fibroblasts to functional endothelial cells. Here, we engineered hESCs to ectopically express hETV2 to create in vitro vasculature in hCOs, namely vhCOs (vascularized hCOs). hETV2-expressing cells in hCOs contributed to forming a complex vascular network in hCOs. Importantly, the presence of vascularization resulted in enhanced functional maturation of organoids. We found that vhCOs acquired several blood-brain barrier (BBB) characteristics including increased expression of tight junctions, nutrient transporters, and trans-endothelial electrical resistance. Finally, hETV2-induced endothelium supported the formation of perfused blood vessels in vivo. These vhCOs form vasculature that resemble early prenatal brain, and present a robust model to study brain disease in vitro.

2019-09-19 | GSE134049 | GEO

Analysis of gene expression in mouse brain regions after exposure to pulse-modulated 1.9 GHz radiofrequency fields

Project description:This study assessed changes in gene expression in 7 mouse brain regions using Illumina Mouse WG-6 (v2) beadchips after exposure to 1.9 GHz pulse-modulated radiofrequency field exposure for 4h/day for 5 days. High dose RF field exposure was whole body average SAR and brain average SAR were 1.45 W/kg and 0.16 W/kg, respectively. Total RNA isolated from the amygdala, auditory cortex, caudate, cerebellum, hippocampus, hypothalamus and medial prefrontal cortex of 1.9 GHz pulse-modulated radiofrequency field exposed mice and compared to that in sham (handled) exposed animals. An unhandled control group was also included in the study design. A total of 20 RNA samples (5 independent biological tissues for each of 4 treatment groups) were collected for each brain region. When analysis of gene expression was conducted within individual brain regions when controlling the false discovery rate (FDR), no differentially expressed genes were identified relative to the sham control. However, it must be noted that most fold changes among groups were observed to be less than 1.5 fold and this study had limited ability to detect such small changes. While some genes were differentially expressed without correction for multiple-comparisons testing, no consistent pattern of response was observed among different RF-exposure levels or among different RF-modulations. The current study provides the most comprehensive analysis of potential gene expression changes in the rodent brain in response to RF field exposure conducted to date. Within the exposure conditions and limitations of this study, no convincing evidence of consistent changes in gene expression was found in response to 1.9 GHz pulse-modulated RF field exposure.

2016-04-22 | E-MTAB-4674 | biostudies-arrayexpress

Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz continuous-wave radiofrequency fields

Project description:This study assessed changes in gene expression in 7 mouse brain regions using Illumina Mouse WG-6 (v2) beadchips after exposure to 1.9 GHz continuous-wave radiofrequency field exposure for 4h/day for 5 days. High dose RF field exposure was whole body average SAR and brain average SAR were 1.36 W/kg and 0.19 W/kg, respectively. Total RNA isolated from the amygdala, caudate, cerebellum, hippocampus, hypothalamus and medial prefrontal cortex of 1.9 GHz continuous-wave radiofrequency field exposed mice and compared to that in sham (handled) exposed animals. An unhandled control group was also included in the study design. A total of 20 RNA samples (5 independent biological tissues for each of 4 treatment groups) were collected for each brain region. When analysis of gene expression was conducted within individual brain regions when controlling the false discovery rate (FDR), no differentially expressed genes were identified relative to the sham control. However, it must be noted that most fold changes among groups were observed to be less than 1.5 fold and this study had limited ability to detect such small changes. While some genes were differentially expressed without correction for multiple-comparisons testing, no consistent pattern of response was observed among different RF-exposure levels or among different RF-modulations. The current study provides the most comprehensive analysis of potential gene expression changes in the rodent brain in response to RF field exposure conducted to date. Within the exposure conditions and limitations of this study, no convincing evidence of consistent changes in gene expression was found in response to 1.9 GHz pulse-modulated RF field exposure.

2016-04-22 | E-MTAB-4675 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data