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HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [iCLIP]

ABSTRACT: To investigate the RNA binding landscape of HNRNPH, we performed an individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) experiment for HNRNPH in MCF7 cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE303135 | GEO | 2026/04/14

REPOSITORIES: GEO

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HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [iCLIP]

Project description:HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [iCLIP]

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Project description:To investigate alternative splicing regulation mediated by HNRNPH, we gradually decreased and increased HNRNPH protein concentration in MCF7 cells. Then, we performed splicing analysis using the data from the RNA-Seq.

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HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [in vitro iCLIP]

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HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [in vitro iCLIP]

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HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale

Project description:This SuperSeries is composed of the SubSeries listed below.

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HNRNPH controls alternative splicing cooperatively on a transcriptome-wide scale [RTstop profiling]

Project description:High-throughput experimental validation of G-quadruplex formation for RNAs with HNRNPH binding sites is not available. Hence, we performed an in vitro Reverse Transcriptase Stop-Profiling (RT-Stop profiling) in high-throughput to map G-quadruplex structure formation of 2000 individual RNAs that have been identified to be bound by HNRNPH.

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iCLIP and NGS to map protein-RNA interactions for U1-snRNP proteins in Trypanosoma brucei

Project description:Individual-nucleotide resolution UV-crosslinking and immunoprecipitation (iCLIP) combined with high-throughput sequencing was performed to generate genome-wide binding maps of two U1-snRNP proteins: U1C and U1-70K in Trypanosoma brucei. 3 (2) biological replicates of U1C (U1-70K) -specific co-immunoprecipitated RNA after UV-crosslinking

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