Dataset Information

Nano MiRNA-Functionating Tetrahedral Framework Nucleic Acid for Cartilage-targeted Ferritinophagy Modulation to Attenuate Temporomandibular Joint Osteoarthritis

ABSTRACT: Background and objectives: Current clinical interventions lack effective strategies to arrest temporomandibular joint osteoarthritis (TMJOA) progression. Emerging evidence highlights the therapeutic potential of microRNAs (miRNAs) in osteoarthritis (OA) management, though critical challenges persist regarding delivery efficiency, including unsatisfactory cellular uptake, immunogenicity, and structural instability of miRNA-based therapeutics. Methods and results: Considering the powerful editability of tetrahedral framework nucleic acids (tFNAs) for gene delivery, we engineered a novel nanoscale gene delivery system using tetrahedral framework nucleic acids functionalized with miR-143-3p (tFNAs-143). Through comprehensive in vivo modeling of TMJOA destabilization and in vitro simulation of IL-1β-induced inflammatory microenvironments, we systematically investigated the therapeutic efficacy and molecular mechanisms underlying OA pathophysiology of tFNAs-143. Our results demonstrated that tFNAs-143 exhibited excellent cellular internalization in chondrocytes and effectively mitigated TMJOA progression by impeding ferritinophagy-mediated ferroptosis. Conclusions: This study advanced miRNA delivery technology for TMJOA therapy, deepened the insights into TMJOA pathogenesis, and proposed a promising nano therapeutic strategy for developing targeted TMJOA therapies.

ORGANISM(S): Mus musculus

PROVIDER: GSE303194 | GEO | 2026/01/21

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Free extracellular DNA (exDNA) accumulation within the cytoplasm is closely associated with chronic inflammation in intervertebral disc degeneration (IVDD). While numerous minimally invasive treatments targeting the various etiologies of IVDD have been developed, there remains a lack of systematic approaches to address the chronic inflammation caused by both intracellular and extracellular nucleic acids. In this study, we propose a novel strategy for treating IVDD that involves dual nucleic acid clearance. This approach targets and regulates mitochondrial DNA (mtDNA) release while clearing exDNA from the microenvironment, working synergistically to block the inflammatory cascade. We first analyzed the mechanism of chronic inflammation mediated by intracellular and extracellular nucleic acids during the progression of IVDD using single-cell sequencing and clinical sample analysis. Next, we developed a composite delivery system consisting of engineered nanovesicles with mitochondrial targeting functions (BNPMT) and a nucleic acid clearance hydrogel (H-P gel). BNPMT achieves targeted delivery to nucleus pulposus cells via membrane modification with triphenylphosphonium (TPP), while encapsulated BAI1, a small molecule drug, specifically inhibits mitochondrial outer membrane permeabilization (miMOMP). This reduces mitochondrial DNA (mtDNA) leakage into the cytoplasm, thereby blocking the mtDNA-cGAS/STING signaling pathway-mediated inflammatory response in nucleus pulposus cells. The H-P gel, crosslinked by Schiff base reactions and using G3-PAMAM dendrimers as functional crosslinkers, efficiently captures exDNA from the intervertebral disc microenvironment. This inhibits cfDNA-induced M1 macrophage polarization and the release of inflammatory cytokines. In an IVDD rat model, this dual-modal system demonstrated synergistic therapeutic advantages, significantly reversing the progression of IVDD over 8 weeks. This targeted nucleic acid clearance strategy provides a novel approach for treating IVDD and offers a theoretical framework for addressing other nucleic acid-related inflammatory diseases.

Dataset Information

Nano MiRNA-Functionating Tetrahedral Framework Nucleic Acid for Cartilage-targeted Ferritinophagy Modulation to Attenuate Temporomandibular Joint Osteoarthritis

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets