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Trajectories and Influencing Factors of Psychological Distress in Nasopharyngeal Carcinoma Patients Receiving Radiotherapy (Incorporating Genetic Factors): A Multicenter Longitudinal Study

ABSTRACT: Background: This multicenter longitudinal study seeks to investigate the dynamic changes of psychological distress (PD) in nasopharyngeal carcinoma (NPC) patients during radiotherapy, and reveal the expression profiles and regulatory networks of circRNAs in these NPC patients. Methods: 282 newly diagnosed NPC patients from three hospitals in China were included. Participants completed questionnaires and provided blood samples. PD trajectories were identified via a latent class growth model (LCGM). Moreover, the factors that influence the PD trajectories were explored. Whole transcriptome sequencing was performed to investigate genetic factors. The real-time quantitative PCR was applied to validate circRNAs. We predicted the target mirnas, target Mrnas and target RNA-binding proteins (RBPs) of the top 10 malregulated circrnas. Subsequently, circRNA-miRNA-mRNA (ceRNA) and circRNA-RBP networks were constructed. In addition, the role of circRNA and target mRNA parent genes was predicted by KEGG and GO analysis. Results: LCGM identified two of the most important PD trajectories during radiotherapy in NPC patients: Class 1 “decline distress group” (11.0%) and Class 4 “rise distress group” (20.3%). Household monthly per capita income, coping strategies, and perceived social support emerged as important predictors of PD trajectories. Regarding genetic factors, 600 circRNAs and 123 miRNAs were identified as being significantly differentially expressed. Notably, hsa_circ_0004277 demonstrated significant differences between patients in the rise and decline distress groups (P < 0.01). ceRNA and RBP networks may influence the pathophysiology of PD in NPC patients undergoing radiotherapy. Conclusion: This study unraveled that PD trajectories in NPC patients during radiotherapy were heterogeneous, indicating the need for screening and timely interventions within this population. Furthermore, the expression patterns of ceRNA and circRNA–RBP networks and pathways related to these networks suggested a potential role of circRNAs in developing PD among NPC patients receiving radiotherapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE303758 | GEO | 2025/09/17

REPOSITORIES: GEO

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Project description:Background: Of Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), 85%-90% of cases develop from liver cirrhosis, and circular RNAs (circRNAs) have important roles in this process; however, differences in serum circRNA expression profiles between patients with HBV-related cirrhosis and those with HCC have not been studied. Methods: Serum RNA was extracted from patients with HCC and cirrhosis (n = 5 per group) and used for microarray analysis of circRNA expression profiles. Bioinformatics analyses, including clustering, differential expression, and construction of a ceRNA network, were performed. Quantitative real-time reverse transcription PCR validation analysis was conducted using samples from patients with HBV-related cirrhosis (n = 88) and HCC (n = 73). Further, statistical analyses were used to analyze the potential function and value of selected circRNAs with expression differing between the HBV-related cirrhosis and HCC groups. Results: Cluster analysis revealed 8 up-regulated and 80 down-regulated circRNAs. Further, qRT-PCR analysis showed that circRNA_0000367 expression was consistent with that detected by microarray experiments, with significantly lower levels in patients with HBV-related HCC than those with HBV-related cirrhosis. CircRNA_0000367 expression levels were also significantly lower in patients with drug-resistant HBV and those with HBV-related cirrhosis with Model for End-Stage Liver Disease score < 10. Further, circRNA_00000367 expression levels were lower in patients with HBV-related cirrhosis who progressed to HCC. Analysis of the lncRNA-miRNA-mRNA ceRNA network identified 39 miRNAs and 24 mRNAs involved in circRNA_00000367 networks; these target genes were involved in various biological processes and signaling pathways. Conclusion: Serum cicrRNA_0000367 is a potential HCC biomarker in patients with HBV-related cirrhosis, where down-regulation of cicrRNA_0000367 in HBV-related cirrhosis may be associated with progression to HCC.

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Trajectories and Influencing Factors of Psychological Distress in Nasopharyngeal Carcinoma Patients Receiving Radiotherapy (Incorporating Genetic Factors): A Multicenter Longitudinal Study

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