Human iPSC-Derived Spinal Neural Progenitors Enhance Sensorimotor Recovery in Spinal Cord-Injured NOD-SCID Mice via Differentiation and Microenvironment Regulation

ABSTRACT: We developed a therapeutic approach by transplanting spinal neural progenitor cells (spNPGs), derived from human induced pluripotent stem cell (iPSC)-generated neuromesodermal progenitors, into a contusive spinal cord injury (SCI) model in NOD-SCID mice. Single-cell RNA sequencing mapped the in vitro differentiation of iPSC-spNPGs, confirming their specification into spinal neuronal lineages. Single-nucleus transcriptomics at one-week post-transplantation showed that the grafted cells differentiated in vivo into motor neurons and two interneuron subtypes (V2 and dI4).

ORGANISM(S): Homo sapiens

PROVIDER: GSE303787 | GEO | 2025/08/05

REPOSITORIES: GEO