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An unbiased survey of distal element-gene regulatory interactions with direct-capture targeted Perturb-seq

ABSTRACT: We developed Direct-Capture Targeted Perturb-seq (DC-TAP-seq), a cost-effective and sensitive approach that integrates U6-driven guide RNA capture with TAP-seq, enabling high-throughput screening of thousands of E–G connections at lower read depth. We applied this method to perform unbiased enhancer screens in two human cell types, K562 and WTC11. Our dataset revealed novel classes of non-canonical cis-regulatory elements (CREs) that were under-sampled in previous CRISPRi screens

ORGANISM(S): Homo sapiens

PROVIDER: GSE303901 | GEO | 2025/08/15

REPOSITORIES: GEO

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