JAX Study 10- RNA-seq of male KOLF2.2J hiPSC-derived endoderm and mesoderm homozygous null for three different transcription factors.

ABSTRACT: This study aims to phenotype iPSC-derived early endoderm and mesoderm that contain homozygous null alleles for the following transcription factors: ISL1, NKX2-1, and EOMES. Null alleles were generated using insertion of a premature termination codon with frameshift (PTC+1) in the case of ISL1 or full protein coding region deletion (KO) for NKX2-1 and EOMES. RNA-seq data was generated after 5 days of initiating differentiation from iPSCs. This analysis allowed the investigation of early embryonic development, cell fate mapping, and lineage analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE303919 | GEO | 2025/09/01

REPOSITORIES: GEO