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Effects of attenuating astrocytic Ca2+ activity in the medial prefrontal cortex with iβARK on transcriptional profiles in 5xFAD mice (snRNA-seq)

ABSTRACT: We injected adeno-associated viruses (AAV2/5) carrying an inhibitory peptide derived from β-adrenergic receptor kinase 1 (iβARK), tagged with mCherry for identification, into the medial prefrontal cortex (mPFC) of 5xFAD mice. This strategy aimed to attenuate Gq GPCR-induced Ca2+ activity in astrocytes. In the control group, mice were injected with AAV2/5 expressing only mCherry. Subsequently, snRNA sequencing analysis was conducted on the mPFC tissue samples from these mice, enabling a comparative assessment of transcriptomic alterations and an exploration of the potential implications resulting from the modulation of Gq-GPCR signaling.

ORGANISM(S): Mus musculus

PROVIDER: GSE304073 | GEO | 2025/10/20

REPOSITORIES: GEO

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| PRJNA1298868 | ENA

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