Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Dual Role of CREPT in Glioblastoma: Promoting Proliferation and Shaping an Immunosuppressive Microenvironment through Chemo-MO and GZMK⁺ T Cell Interaction

ABSTRACT: Elevated CREPT promotes tumor cell proliferation and enhances CCL2 secretion, thereby recruiting Chemo-MOs. These Chemo-MOs then engage in regulatory interactions with GZMK+CD8+ T cells, suppressing T cell infiltration and ultimately establishing a robust immunosuppressive microenvironment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE304176 | GEO | 2025/08/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The Dual Role of CREPT in Glioblastoma: Promoting Proliferation and Shaping an Immunosuppressive Microenvironment through Chemo-MO and GZMK⁺ T Cell Interaction

Project description:The Dual Role of CREPT in Glioblastoma: Promoting Proliferation and Shaping an Immunosuppressive Microenvironment through Chemo-MO and GZMK⁺ T Cell Interaction

| PRJNA1299322 | ENA

ChIP-seq of CREPT in intestinal crypts

Project description:CREPT was demonstrated as a key regulator of intestinal stem cells. To figure out the role of CREPT on transcription level, a ChIP-seq experiment was performed to identify the CREPT down-stream genes in intestinal crypt cells.

2020-11-08 | GSE158243 | GEO

Gene expression profiles of WT and CREPT deleted Lgr5+ intestinal stem cells

Project description:Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. The Lgr5+ ISCs have much higher CREPT protein level than Lgr5- cells. To explore the function of CREPT in ISCs, we isolated WT and CREPT deleted Lgr5+ ISCs (Lgr5-CREPTKO) to perform Next generation sequencing.

2020-11-08 | GSE143604 | GEO

Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization

Project description:Cancer local recurrence increases the mortality of patients, and might be caused by field cancerization, a pre-malignant alteration of normal epithelial cells. It has been suggested that cancer-derived small extracellular vesicles (CDEs) may contribute to field cancerization, but the underlying mechanisms remain poorly understood. In this study, we aim to identify the key regulatory factors within recipient cells under the instigation of CDEs. In vitro experiments were performed to demonstrate that CDEs promote the expression of CREPT in normal epithelial cells. TMT-based quantitative mass spectrometry was employed to investigate the proteomic differences between normal cells and tumor cells. Loss-of-function approaches by CRISPR-Cas9 system were used to assess the role of CREPT in CDEs-induced field cancerization. RNA-seq was performed to explore the genes regulated by CREPT during field cancerization. CDEs promote field cancerization by inducing the expression of CREPT in non-malignant epithelial cells through activating the ERK signaling pathway. Intriguingly, CDEs failed to induce field cancerization when CREPT was deleted, highlighting the importance of CREPT. Transcriptomic analyses revealed that CDEs elicited inflammatory responses, primarily through activation of the TNF signaling pathway. CREPT, in turn, regulates the transduction of downstream signals of TNF by modulating the expression of TNFR2 and PI3K, thereby promoting inflammation-to-cancer transition. CREPT not only serves as a biomarker for field cancerization, but also emerges as a target for preventing the cancer local recurrence.

2023-12-10 | GSE249383 | GEO

Gene expression profiles of irradiated WT and CREPT deleted intestinal epithelium

Project description:Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. The Lgr5+ ISCs have much higher CREPT protein level than Lgr5- cells. To explore the function of CREPTduring regeneration, we isolated irradiated WT and CREPT deleted intestines (Vil-CREPTKO) to perform Next generation sequencing.

2020-11-08 | GSE143605 | GEO

Single cell gene expression profile of irradiated small intestinal crypt cells of WT and CREPT KO mice

Project description:To investigate the role of CREPT in intestinal regeneration, we performed single cell RNA sequencing (scRNA-seq) analyses on irradiated small intestinal crypt cells of WT and CREPT KO mice to figure out the change of different types of intestinal epithelial cells.

2025-07-07 | GSE228700 | GEO

Analysis of MO ultrafiltrates from different rabbit tissues

Project description:We analysed MOs of different organs from rabbit for comparative analysis with relative quantification using LFQ.

2025-07-14 | PXD051701 | Pride

ChIP-seq of CREPT in intestinal crypts

Project description:ChIP-seq of CREPT in intestinal crypts

| PRJNA664508 | ENA

Single-cell RNA-seq profiling of treatment-naïve chemo-refractory and chemo-sensitive ovarian cancer

Project description:This dataset contains single-cell RNA-seq data from treatment-naïve biopsies of nine patients diagnosed with ovarian high-grade serous carcinoma (HGSC), including four chemo-refractory and five chemo-sensitive cancers. Four of the samples (EOC204, EOC115, EOC649, EOC1127) are newly generated, while five samples have been previously published (GSE165897). The data was generated as part of the DECIDER observational trial (NCT04846933) to investigate the molecular drivers of chemo-refractory HGSC. Key findings from the study indicate that chemo-refractory HGSC is associated with reduced interferon type I (IFN-I) activity and enhanced hypoxia pathway activity, while baseline IFN-I pathway activity in chemo-naïve cancer serves as an independent prognostic factor for chemotherapy response.

2025-07-03 | GSE300897 | GEO

Expression data on human MO-to-DC and MO-to-MAC differentiation

Project description:Comparison of the RNA expression profiles of CD14+ monocytes from human peripheral blood with derived dendritic cells (DCs) and macrophages (MACs) obtained by exposure with GM-CSF/IL-4 and GM-CSF, respectively, and with mature DCs and MACs after lipopolysaccharide (LPS) exposure The expression profiles of RNA of human CD14+ monocytes were compared with derived immature dendritic cells (iDCs) and macrophages (iMACs) following GM-CSF/IL-4 and GM-CSF incubation, and then activation/maturation with lypopolysaccharyde (LPS) using the Affymetrix PrimeView Human Gene Expression array (Affymetrix, Santa Clara, CA). This platform allows the interrogation of >36,000 transcrits and variants per sample. The samples were hybridized in the array following the manufacturerâ??s instructions. Total RNA isolated by standard procedures from CD14+ cells (total monocytes, MOs) corresponding to three sets of samples of monocytes (MOs), derived immature DCs and MACs (iDCS and iMACS) and activated/mature DCs and MACs following incubation with LPS (mDCS and mMACs)

2015-12-12 | E-GEOD-75938 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data