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Embryogenesis col0-somatic embryogenesis

ABSTRACT: gnp07_regeneome_embryogenesis - embryogenesis col0 - Identify genes involved in somatic embryogenesis - compare embryogenic areas of a callus with undifferenciate area in the same callus

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE30425 | GEO | 2012/06/01

SECONDARY ACCESSION(S): PRJNA143393

REPOSITORIES: GEO

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embryogenesis col0-somatic embryogenesis

Project description:gnp07_regeneome_embryogenesis - embryogenesis col0 - Identify genes involved in somatic embryogenesis - compare embryogenic areas of a callus with undifferenciate area in the same callus 4 dye-swap - tissue comparison

2012-05-31 | E-GEOD-30425 | biostudies-arrayexpress

embryogenesis ws-somatic embryogenesis

Project description:gnp07_regeneome_embryogenesis - embryogenesis ws - Identify genes involved in somatic embryogenesis - To compare embryogenic areas of a callus with undifferenciate area in the same callus

2012-06-01 | GSE30383 | GEO

embryogenesis ws-somatic embryogenesis

2012-05-31 | E-GEOD-30383 | biostudies-arrayexpress

embryogenesis ws-somatic embryogenesis

2013-04-11 | E-GEOD-45940 | biostudies-arrayexpress

small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis [RNA-Seq]

Project description:We report the role of sRNAs populations during the induction of callus tissues from VS-535 maize embryos displaying contrasting in vitro embryogenic potential; characterized through Next-generation sequencing (NGS). We conclude that the Embryogenic Response during Maize Somatic Embryogenesis induction is closely related to sRNAs regulation and depends on the developmental stage of the explant.

2019-10-21 | GSE120435 | GEO

small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis [smRNA-Seq]

2019-10-21 | GSE120436 | GEO

Somatic embryogenesis of Pinus nigra

Project description:There are several issues complicating somatic embryogenesis in conifers, for instance the low initiation frequency, the weak maturation capacity, and the loss of an embryogenic potential after a prolonged cultivation. The aim of our study was to clarify molecular details of the this process in the tree Pinus nigra Arn. We performed comparative proteomic analysis based on the two-dimensional gel electrophoresis in 2 genotypes coded 362 and 366 of: 1) proliferating embryogenic tissues (E) initiated from immature zygotic embryos, 2) non-embryogenic calli (NEC) started from cotyledons of somatic seedlings, 3) embryogenic tissues that lost the maturation capacity (E-L). Pine tissues showed distinct morphologic features: a) E362 and E366 were characterized by the presence of early bipolar structures capable of maturation and plantlet regeneration; b) NEC362 and NEC366 were composed of round shaped cells without any organisation; c) for E-L362 and E-L366 the long-term culture of initially embryogenic tissues resulted in the disorganisation of early bipolar structures. We found 24 differentially accumulated protein spots common for both genotypes after comparison of E versus NEC.

2017-09-06 | PXD004611 | Pride

Repetitive somatic embryogenesis induced cytological and proteomic changes in propagated embryogenic lines of Pseudotsuga menziesii [Mirb.]

Project description:Objectives: to characterize and to better understand changes at the cellular and molecular levels, in embryogenic lines in Douglas-fir obtained after repetitive somatic embryogenesis, that could explain improvement of their embryogenic potential : to primary lines, secondary ones showed an increase of their embryogenic potential; then tertiary lines showed again an improved embryogenic potential compared to second lines. Interestingly, the proteomic analysis further revealed different sets of significant proteins suggesting that each cycle of repetitive somatic embryogenesis is promoting substantial genome-wide rearrangement of the gene expression pattern.

2018-08-15 | PXD008347 | Pride

Embryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity

Project description:Somatic embryogenesis is an important biological process in several plant species, including sugarcane. Proteomics approaches have shown that H+ pumps are differentially regulated during somatic embryogenesis; however, the relationship between H+ flux and embryogenic competence is still unclear. This work aimed to elucidate the association between extracellular H+ flux and somatic embryo maturation in sugarcane. We performed a microsomal proteomics analysis and analyzed changes in extracellular H+ flux and H+ pump (P-H+-ATPase, V-H+-ATPase and H+-PPase) activity in embryogenic and non-embryogenic callus. A total of 657 proteins were identified, 16 of which were H+ pumps. We observed that P-H+-ATPase and H+-PPase were more abundant in embryogenic callus. Compared with non-embryogenic callus, embryogenic callus showed high H+ influx, especially at maturation day 14 as well as higher H+ pump activity, mainly P-H+-ATPase and H+-PPase activity. The H+-PPase appears to be the major H+ pump in embryogenic callus during somatic embryo formation, functioning in both vacuole acidification and PPi homeostasis. These results provide evidence for an association between higher H+ pump protein abundance and, consequently, higher H+ flux and embryogenic competence acquisition in the callus of sugarcane.

2018-06-26 | PXD009858 | Pride

MicroRNA expression during somatic embryogenesis in Arabidopsis

Project description:The microarray test aims to find the miRNAs involved in somatic embryogenesis in Arabidopsis. The plant microRNA array V2.0 (CapitalBio Corp., Beijing, China) contained 426 non-reduplicated plant miRNA probes, including 188 in Arabidopsis. Finally, 75 plant miRNAs expressed differentially, 36 increased and 39 decreased included. Two critical period samples of somatic embryogenesis were chosen for the microarray test: the edge of embryonic calli in embryonic callus-inducing medium (ECIM) for 14 days and the secondary somatic embryos protuberances in somatic embryo-inducing medium (SEIM) for 2 days (marked as “14D” and “J2D” accordingly) . Three chip were test in each sample.

2012-02-13 | E-GEOD-35759 | biostudies-arrayexpress

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