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Effects of stable SpCas9 expression in affecting gene expression in DU145 cells

ABSTRACT: SpCas9 has been broadly used in gene editing, while as a bacterial protein, its effects on mammalian cell behavior remains unclear. We established stable SpCas9 expression in a panel of mammalian cell lines and found stable SpCas9 expression could affect cell growth in vitro. For example, we observed that SpCas9 expression promoted DU145 cell proliferation. To understand underlying mechansims, we performed this RNA-Seq analysis in DU145-NC (negative control) and SpCas9 expressing cells, aiming to find how SpCas9 modulates gene expression that may contribute to its cell proliferation control.

ORGANISM(S): Homo sapiens

PROVIDER: GSE304365 | GEO | 2025/11/14

REPOSITORIES: GEO

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Effects of stable SpCas9 expression in affecting gene expression in DU145 cells

Project description:Effects of stable SpCas9 expression in affecting gene expression in DU145 cells

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