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Assessment of the role of genetic variation in microRNAs on the susceptibility and physiopathology of Gastric Cancer

ABSTRACT: Gastric Cancer (GC) continues to be a disease with a poor prognosis being a leading cause of cancer death worldwide. Varying incidence among regions depends on several factors such as Helicobacter pylori infection, environmental exposure and genetic background. GC is the result of a multifactorial complex process for which a multistep model of carcinogenesis is currently accepted. The clinical outcome of GC is highly dependent on the local progression and metastasis development, which is partially controlled by oncogenes and tumor suppressor genes, whose expression is further regulated by microRNAs (miRNAs). MiRNAs are non-protein-coding small RNAs that act as post-transcriptional gene repressors participating in the regulation of most biological processes. Several miRNAs have been found differentially expressed between tumor and normal tissues in GC, also circulating miRNAs in serum/plasma have been proposed as a novel class of non-invasive biomarkers with a great potential for early detection and prognostic of cancer. We propose that the analysis of the differential expression of miRNAs in the serum of controls and patients at different disease stages of GC might lead to the discovery of new GC causative factors as well as new non-invasive diagnostic biomarkers for GC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE304450 | GEO | 2026/06/12

REPOSITORIES: GEO

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Project description:Background: Gastric cancer (GC) is often associated with a poor prognosis, due to its asymptomatic early stages. The current gold standard for diagnosing GC, upper endoscopy, is invasive and has limited sensitivity for detecting gastric preneoplasia such as dysplasia. Non-invasive biomarkers, such as circulating proteins in the blood, hold promise for the early detection of asymptomatic gastric lesions. Methods: During an exploratory study, plasma samples from 39 participants, including patients diagnosed with different gastric pathologies (gastritis, preneoplasia, GC) and healthy controls, were analyzed using mass spectrometry-based proteomics. Differentially abundant proteins were identified through pairwise comparisons and sparse Partial Least Squares Discriminant Analysis (sparse PLS-DA). Fifteen candidate proteins were selected and quantified by ELISA in plasma samples from the full cohort of 138 participants. Results: Proteomic profiling identified 691 proteins in plasma samples. Pairwise comparisons highlighted up to 213 candidate biomarkers distinguishing cancer patients from healthy controls, while distinguishing gastritis and preneoplasia proved challenging due to their similar proteomes. Sparse PLS-DA identified 85 proteins that distinguish all patient groups. Subsequent ELISA validation confirmed Leptin as a promising biomarker for detecting gastric preneoplasia, particularly in women. Nine additional proteins (ATAD3B, IGFALS, JUP, LBP, MAN2A1, ARG1, CA2, HPT, KRT14) showed differential plasma levels across patients groups, influenced by age and gender. Multi-biomarker prediction models that incorporated factors such as age, gender, and H. pylori status, outperformed single-protein models. Using 4-fold cross-validation repeated 10 times, the best-performing models achieved high predictive accuracy, with mean AUROC=85.3% for classifications cancer vs. non-cancer and 83.9% for cancer/preneoplasia vs. healthy/gastritis. On the full cohort, the best biomarker combinations reached AUROC values exceeding 94% for these classifications. Conclusions: This study introduces a novel, non-invasive approach for predicting GC based on plasma protein concentrations across a broad spectrum of gastric pathologies. Predictive models, validated through robust cross-validation, demonstrated high accuracy using a limited panel of biomarkers. By relying on simple blood sampling, this strategy holds promise for high-risk gastric mucosal lesions, including at asymptomatic stages. Such an approach could significantly improve early detection and clinical management of GC, offering direct benefit for patients outcomes.

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Assessment of the role of genetic variation in microRNAs on the susceptibility and physiopathology of Gastric Cancer

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