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Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [BiSulfite-seq]

ABSTRACT: Induced resistance against (hemi-)biotrophic pathogens can be triggered in Arabidopsis by 17β-estradiol (E2)-dependent activation of ROS1 in plants carrying the XVE:ROS1-YFP transgene. Here, we characterise the genome-wide cytosine DNA methylation changes associated with the estbalishment of this ROS1-mediated resistance.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE304520 | GEO | 2026/09/10

REPOSITORIES: GEO

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Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [RNA-seq]

Project description:Induced resistance against (hemi-)biotrophic pathogens can be triggered in Arabidopsis by 17β-estradiol (E2)-dependent activation of ROS1 in plants carrying the XVE:ROS1-YFP transgene. Here, we use ribosome-depleted RNA-seq to characterise the transcriptional changes associated with the estbalishment of this ROS1-mediated resistance.

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Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [ONT-seq]

Project description:Induced resistance against (hemi-)biotrophic pathogens can be triggered in Arabidopsis by 17β-estradiol (E2)-dependent activation of ROS1 in plants carrying the XVE:ROS1-YFP transgene. Here, we use long-read Oxford Nanopore Technologies sequencing (ONT-seq) and the centromere-complete Arabidopsis genome assembly Col-CEN to characterise genome-wide changes in DNA methylation associated with the establishment of ROS1-mediated resistance, with a particular focus on pericentromeric and centromeric regions.

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Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [BiSulfite-seq]

Project description:Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [BiSulfite-seq]

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Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [RNA-seq]

Project description:Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [RNA-seq]

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Project description:Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [ONT-seq]

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Project description:Contrasting epigenetic dynamics in chromosome arms and pericentromeres during ROS1-induced plant immune memory [small RNA-seq]

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Project description:To survey transcriptome changes by the mutations of a DNA demethylase ROS1 responding to a phytohormone abscisic acid, we performed the Next-gen sequencing (NGS) associated RNA-seq analysis. Two ROS1 knockout lines (ros1-3, ros1-4; Penterman et al. 2007 [PMID: 17409185]) with the wild-type Col line (wt) were subjected. Three samples (ros1-3, ros1-4 and wt), biological triplicates, ABA or mock treatment, using Illumina HiSeq 2500 system

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