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Redistribution of RNA localization in early embryos of trout hybrid (Oncorhynchus mykiss egg fertilized with Salvelinus fontinalis sperm)

ABSTRACT: This study investigates the spatial transcriptomic dynamics during early embryogenesis in a hybrid trout, where the Oncorhynchus mykiss (rainbow trout) egg was fertilized with the Salvelinus fontinalis sperm. Using TOMO-seq, we profiled RNA localization along the animal–vegetal axis at key embryonic stages, 0 hpf (freshly fertilized egg/zygote), 1 hpf (early cleavage stage), 1 dpf (early blastula stage, ~32-64 cells), and 3 dpf (mid-blastula, ~1000 cells). Samples were collected in biological triplicates and sectioned into two sections (A & B) along the axis to assess spatial distribution. The data were used to analyze for de novo transcription by looking for paternal transcripts. Our results revealed spatially distinct transcriptome changes.

ORGANISM(S): Oncorhynchus mykiss

PROVIDER: GSE304716 | GEO | 2026/01/08

REPOSITORIES: GEO

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