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AoMbp1 interacts with AoSwi6 and plays pleiotropic roles in mycelial development, trap morphogenesis, and stress respone in the nematode-trapping fungus Arthrobotrys oligospora [RNA-Seq]

ABSTRACT: The fungal specific APSES family proteins involve in regulating fungal growth, development, and multiple biological processes. In this study, AoMbp1, an ortholog of Saccharomyces cerevisiae APSES-type transcription factor Mbp1, was functionally analyzed in a representative nematode-trapping fungus Arthrobotrys oligospora. Inactivation of Aombp1 caused a severe affect on the mycelial growth and development, the mycelial growth rate of ∆Aombp1 mutant was remarkably decreased, the hyphal septa were increased whereas the number of nuclei were significantly reduced, and the lipid droplet accumulation was remarkably increased. Meanwhile, the deletion of Aombp1 resulted a considerable reduction in the number of conidiophores and spore yield, which also caused abnormal spore morphology. In addition, the ∆Aombp1 mutants became more sensitive to several chemical stressors, especially to hyperosmotic reagents. Importantly, disruption of Aombp1 caused the number of traps and nematode-trapping ability were significantly reduced, and most of the traps have changed from their original three-dimensional structure to a planar shape. RNA-Seq, DAP-Seq and Y2H assay showed that AoMbp1 interacted with AoSwi6, and involved in regulating cell cycle, meiosis, lipid metabolism, DNA replication, mismatch repair and nucleotide excision repair. Our study elucidated the functions and potential regulatory mechanism of APSES protein Mbp1 in the mycelial development and trap morphogenesis of nematode-trapping fungi.

ORGANISM(S): Orbilia oligospora

PROVIDER: GSE305351 | GEO | 2025/09/11

REPOSITORIES: GEO

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Project description:As a dynamic structure with a barrier between the cell and the outside world, maintenance of cell wall integrity (CWI) is important for fungal growth, development and pathogenicity processes. Here we characterized a MADS-box transcription factor RlmA (AoRlmA) downstream of the CWI regulatory pathway in the nematode-trapping fungus Arthrobotrys oligospora. Deletion of AorlmA caused a reduction in mycelial growth and the number of nuclei, and significant downregulation of transcript levels of genes related to nucleus synthesis and DNA damage repair, such as rad3, spo11, and rad25. Meanwhile, the ΔAorlmA mutant strains showed a significant reduction in spore production compared with the wild-type (WT) strain, and the transcript levels of sporulation-related genes was downregulated in the ΔAorlmA mutant during the early and middle stages of condiation, such as flbA, medA, and vosA. Meanwhile, the mycelial cell wall of the ΔAorlmA mutant strain showed breakage. Also, the transcript levels of genes related to cell wall synthesis were significantly down-regulated in the mutant strain compared to the WT strain, and the mutant strain was more sensitive to stresses of cell wall synthesis disruptors, oxidative stress and osmotic stress than the WT strain. Compared with the WT strain, the ΔAorlmA mutant strain not only had a reduced number of traps and nematicidal ability, but also, the shape of the traps of the mutants has also changed. In addition, In addition, AoRlmA also regulates autophagy and endocytosis. Transcriptome analysis of ΔAorlmA mutant strains showed that AorlmA regulates redox, cell wall synthesis, DNA replication and damage repair, and pathogenic processes. Metabolomic analysis showed that AorlmA is involved in the biosynthesis of secondary metabolites of A. oligospora. To summarise, our data suggest that AorlmA is involved in growth, sporulation, spore germination, maintenance of cell wall integrity, DNA replication and damage repair, pathogenesis, autophagy and secondary metabolite biosynthesis processes.

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AoMbp1 interacts with AoSwi6 and plays pleiotropic roles in mycelial development, trap morphogenesis, and stress respone in the nematode-trapping fungus Arthrobotrys oligospora [RNA-Seq]

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