Nanostraw Electroporation for Temporal RNA Sampling from Living 2D and 3D Cell Culture Systems - RNA sequencing data

ABSTRACT: Traditional gene expression studies extract RNA through destructive cell lysis, restricting analysis to single timepoints and requiring parallel samples. This limits the ability to track transcriptomic changes within the same cell population and poses challenges for experiments with scarce primary samples. To address this, we developed a minimally perturbative, unbiased RNA sampling approach — nano-electroextraction (NEE) — which uses hollow nanostraw membranes with mild electroporation to extract intracellular RNA without compromising cell viability or gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE305507 | GEO | 2026/08/14

REPOSITORIES: GEO