Immune and Transcriptional Landscape of Endometrial Receptivity at P+5 Phase: A Triad Analysis of EIN, RIF, and Control Endometrial Profiles

ABSTRACT: This study delineates the immune and transcriptional landscape of endometrial receptivity at the P5 phase via triad analysis of Endometrial Intraepithelial Neoplasia/Early Endometrial Cancer (EIN/ECa, N = 64), Repeated Implantation Failure (RIF, N = 25), and control (Ctrl., N = 3)) endometrial profiles. RNA sequencing and deconvolution algorithms identified differential gene expression, immune cell composition, and pathway enrichments. EIN/ECa exhibited dysregulated complement/coagulation cascades and proliferation-related genes, suggesting a pro-proliferative/inflammatory microenvironment. RIF showed immune pathway perturbations, with abnormal TLR4 and CXCL10 expression disrupting maternal-fetal tolerance. Integrative analysis revealed distinct molecular signatures: EIN/ECa prioritized oncogenic proliferation, while RIF focused on immune tolerance defects.These findings illuminate phase-specific endometrial receptivity mechanisms, providing targets for improving fertility outcomes in high-risk groups.

ORGANISM(S): Homo sapiens

PROVIDER: GSE305811 | GEO | 2025/08/25

REPOSITORIES: GEO