Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic data of inhibition of CDK12 in GBM12 cell line by SR-4835 treatment

ABSTRACT: This dataset contains transcriptomic profiles of the human glioblastoma cell line GBM12 following treatment with SR-4835, a selective CDK12 inhibitor. GBM12 cells were treated with SR-4835 or vehicle control for 24 hours, and total RNA was extracted for RNA sequencing. The study aims to elucidate transcriptional changes associated with CDK12 inhibition in glioblastoma and to identify downstream targets or pathways affected by SR-4835.

ORGANISM(S): Homo sapiens

PROVIDER: GSE306109 | GEO | 2025/08/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic changes after CDK12/13 inhibition by SR-4835 in ovarian cancer cell lines

Project description:CDK12/13 are transcriptional cyclin-dependent kinases shown to phosphorylate the C-terminal domain of RNA polymerase II. Here, we investigated the changes on the transcriptome level after inhibition of CDK12/13 by the specific inhibitor SR-4835 in a panel of ovarian cancer cell lines: SK-OV-3, A2780, A2780 cis, Caov-3, Caov-3 cis. All cell lines were treated for 24h with 90 nM SR-4835 prior RNA extraction and paired-end RNA sequencing. Differential gene expression, Ingenuity pathway analysis and genes with alternative exon usage was determined.

2025-10-28 | E-MTAB-15987 | biostudies-arrayexpress

Transcriptomic data of inhibition of CDK12 in GBM12 cell line by SR-4835 treatment

Project description:Transcriptomic data of inhibition of CDK12 in GBM12 cell line by SR-4835 treatment

| PRJNA1309139 | ENA

Discovery and design of molecular glue enhancers of CDK12-DDB1 interactions for targeted degradation of cyclin K

Project description:The CDK12 inhibitor, SR-4835, promotes the proteasomal degradation of cyclin K, contingent on the presence of CDK12 and the CUL4-RBX1-DDB1 E3 ligase complex. The inhibitor displays molecular glue activity, which correlates with its enhanced ability to inhibit cell growth. This effect is achieved by facilitating the formation of a ternary complex that requires the small molecule SR-4835, CDK12, and the adaptor protein DDB1, leading to the subsequent ubiquitination and degradation of cyclin K. We have successfully solved the structure of the ternary complex, enabling the de novo design of molecular glues that transform four different CDK12 scaffold inhibitors, including the clinical pan-CDK inhibitor dinaciclib, into cyclin K degraders. These results not only deepen our understanding of CDK12's role in cell regulation but also underscore significant progress in designing molecular glues for targeted protein degradation, paving the way for more precise and effective therapeutic strategies in cancer treatment.

2025-05-07 | PXD053505 | Pride

Heterochromatin remodeling by CDK12 contributes to learning in Drosophila

Project description:We describe the epigenetic profiling of the H3K9me2 and HP1a in Drosophila third instar larvae before and after CDK12 depletion by RNA interference (RNAi). Here we show that CDK12 regulates heterochromatin dynamics in Drosophila chromosomes. Depletion of CDK12 induces the increased HP1a and H3K9me2 binding profile on the coding region of euchromatic genes, with the X chromosome being the most affected. These results are consistent with the polytene chromosome immunostaining pattern of HP1a and H3K9me2 after CDK12 knockdown in our initial cytological observations, which show that CDK12 depletion induce heterochromatin spreading on euchromatic arms, especially on the X chromosome. This study describes a novel role of the CDK12 complex in controlling the epigenetic transition between euchromatin and heterochromatin. Examination of the genome-wide H3K9me2 and HP1a binding profile in wildtype larvae (WT) and CDK12-depleted larvae (CDK12-KD). Examination of the genome-wide CDK12 binding profile in wildtype larvae (WT). Twelve independent immunoprecipitations were conducted for each antibody. Two biological replicates were performed.

2015-11-02 | E-GEOD-63011 | biostudies-arrayexpress

CDK12/13 inhibition in NSCLC cell lines with acquired resistance to sotorasib

Project description:The efficacy of KRAS-G12C inhibitors in lung cancer is limited by the rapid onset of acquired resistance. While divergent mechanisms of resistance across patients and preclinical models have complicated efforts to identify therapeutic strategies for sotorasib-resistant cancers, we found that sotorasib-resistant H358 and LU65 cell lines, which have distinct mechanisms of resistance and drug sensitivity profiles, both exhibited increased vulnerability to treatment with SR-4835, an inhibitor of the transcriptional kinases CDK12 and CDK13. To identify CDK12/13-dependent genes linked to the increased sensitivity we observed in sotorasib-resistant over parental cells, we treated parental and sotorasib-resistant H358 and LU65 cells with SR-4835 and performed RNA sequencing.

2025-10-07 | GSE288707 | GEO

RNA-Seq of Drosophila melanogaster Cdk12-null mutants versus control

Project description:In a forward genetic screen, we have previously identified a null mutant of Cdk12 that results in alterations in actin dynamics, the axon initial segment and electrophysiology in Drosophila melanogaster. To decipher how Cdk12 may be having these effects, we extracted RNA from pooled Drosophila heads and compared Cdk12-null mutants to controls at the transcriptome level.

2023-01-01 | E-MTAB-12090 | biostudies-arrayexpress

modENCODE_White Lab: genome-wide ChIP data of sr from E8-16 on Illumina Genome Analyzer.

Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains genome-wide binding profile of the factor sr from E8-16 generated by ChIP and analyzed on Illumina Genome Analyzer. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

2011-12-16 | GSE34367 | GEO

modENCODE_White Lab: genome-wide ChIP data of sr from E16-24 on Illumina Genome Analyzer.

Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains genome-wide binding profile of the factor sr from E16-24 generated by ChIP and analyzed on Illumina Genome Analyzer. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

2011-12-16 | GSE34358 | GEO

Nascent transcriptome changes in response to CDK12/13 inhibition in gliomas

Project description:To assess changes in the nascent and steady state transcriptomes after treatment with the CDK12/13 inhibitor THZ531, we performed SLAM-seq in the G7 glioblastoma derived cell line after DMSO treatment or treatment with 500 nM THZ531 for 6 hrs.

2026-01-29 | GSE186310 | GEO

SR-4835 in ovarian cancer

Project description:Transcriptomic changes after CDK12/13 inhibition by SR-4835 in ovarian cancer cell lines

| PRJEB93935 | ENA