Dataset Information

Effect of the bile acids chenodeoxycholic acid (CDCA), deoxycholic acid (DCA) and cholic acid (CA) on the transcriptome of Mycobacterium avium strain MAC101

ABSTRACT: The bile acids chenodeoxycholic acid (CDCA), deoxycholic acid (DCA) andcholic acid (CA) were added to the growth medium of cultures of M.avium strain MAC101 and RNA was islated after 1.5 hours or 24 hours of incubation. RNAseq was performed with Illumina sequencing. With a FDR rate threshold of 0.05 showing a large number of genes whose expression was induced by the treatment. The signature sets of induced genes or both bile acids are shown to be very different. there are also common responses in various genes that function in cholesterol catabolism. As a control cholesterol in a similar concentration was used but this had only a very moderate effect on the transcriptome at 1.5 hours.

ORGANISM(S): Mycobacterium avium

PROVIDER: GSE306124 | GEO | 2026/09/01

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Background: Previous epidemiological studies have repeatedly found per- and polyfluoroalkyl substances (PFAS) exposure associated with higher circulating cholesterol, one of the greatest risk factors for development of coronary artery disease. The main route of cholesterol catabolism is through its conversion to bile acids, which circulate between the liver and ileum via enterohepatic circulation. Patients with coronary artery disease have decreased bile acid excretion, indicating that PFAS-induced changes of enterohepatic circulation may play a critical role in cardiovascular risk. Objectives: Using a mouse model with high LDL-VLDL cholesterol and aortic lesion development similar to humans, the current study investigates mechanisms linking exposure to a PFAS mixture with increased cholesterol. Methods: Male and female Ldlr-/- mice were fed an atherogenic diet (Clinton/Cybulsky low fat, 0.15% cholesterol) and exposed to a mixture of 5 PFAS representing legacy, replacement, and emerging subtypes (i.e., PFOA, PFOS, PFNA, PFHxS, GenX), each at a concentration of 2 mg/L, for 7 weeks. Blood was collected longitudinally for cholesterol measurements and mass spectrometry was used to measure circulating and fecal bile acids. Transcriptomic analysis of ileal samples was performed via RNA-sequencing. Results: After 7 weeks of PFAS exposure, average circulating PFAS levels were measured at 21.6, 20.1, 31.2, 23.5, and 1.5 µg/mL in PFAS-exposed females and 12.9, 9.7, 23, 14.3, and 1.7 µg/mL in PFAS-exposed males for PFOA, PFOS, PFHxS, PFNA, and GenX, respectively. PFAS exposure led to elevated circulating cholesterol levels after 7 weeks compared to vehicle mice, with females increasing 18% and males increasing 24%. Total circulating bile acid levels were elevated in PFAS-exposed mice by 230% in males and 290% in females compared to vehicle. PFAS decreased fecal bile acid levels by 62% in females and 59% in males. In the ileum, expression levels of the bile acid reuptake transporter ASBT were increased due to PFAS exposure. Discussion: Mice exposed to a PFAS mixture display increased circulating cholesterol and bile acids perhaps due to changes in enterohepatic circulation. This study implicates PFAS-mediated effects at the site of the ileum as a possible critical mediator of increased cardiovascular risk due to PFAS.

Dataset Information

Effect of the bile acids chenodeoxycholic acid (CDCA), deoxycholic acid (DCA) and cholic acid (CA) on the transcriptome of Mycobacterium avium strain MAC101

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets