Long FAM13A isoforms regulate human lung epithelial development in vitro

ABSTRACT: We performed single cell transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived anterior foregut progenitors (D6) and lung progenitors (D15). FAM13A-long isoforms were knocked out of iPSCs prior to differentiation. Prior to capture, cells were labelled with hashing antibodies (HTO). Cells were captured for 10x Genomics Single cell capture (V3 protocol), and GEX and HTO libraries were sequenced. HTODemux function was used to demultiplex the samples. FAM13A-long KO changed clustering of D15 NKX2-1+ lung progenitors

ORGANISM(S): Homo sapiens

PROVIDER: GSE306210 | GEO | 2026/07/15

REPOSITORIES: GEO