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Ribo-TRAPseq identifies spacially distinct roles for the anterior and posterior intestine for metabolic regulation in C. elegans

ABSTRACT: Animals must couplefood-relatedcuesin the intestinetotheirphysiology, yetit is unclearwhether discrete intestinal segments specialize in this sensing.Here, we generatecelltype-resolved translatomes of the anterior intestinal ring (INT1) and the remaining intestine (INT2-9)inCaenorhabditis elegansusing celltype-specific TRAP-seq under acute and chronicfasting/refeedingconditions. Across states, INT1 shows adistinct identity enriched for immuneand stress-response programs, including C-type lectinsand ShKT-domain families. INT1-targeted perturbations alter bacterial avoidance and survival, indicating specialized immunefunctions. Fasting/refeeding elicit divergent regulatory dynamics; modulating INT1 undulatinggeneschangesthesecretiondynamicsof an INT1-derived enteroendocrine peptide, INS-7.Mechanosensation or bacterial supernatant fails to resetthesecretionof INS-7after fasting,whereasbacteria or pyruvate restore basal levels. Pyruvate requires mitochondrial import andmetabolism;anacute blockade of pyruvate processing prevents refeeding normalization.Thus,we identify INT1 as sentinel enteroendocrine sensors that couple pyruvate-dependentmitochondrial metabolism toenteroendocrine output, providing a resource for segment-specificintestinal biology.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE306302 | GEO | 2025/12/04

REPOSITORIES: GEO

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