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Thre impact of Akkermansia muciniphila on Group B Streptococcus gene expression during infection of human vaginal epithelial cells.

ABSTRACT: Group B Streptococcus (GBS) is an opportunistic pathogen that colonizes the vaginal tract and can be transmitted to the fetus or newborn resulting in infection. Previously we identified that Akkermansia muciniphila alters the dynamics of GBS vaginal colonization. Here we determine that A. muciniphila promotes GBS aggregation and attachment to human vaginal epithelial cells (hVECs). RNA-sequencing analysis revealed that A. muciniphila changed expression of 258 unique GBS genes during hVEC colonization, with many involved in cell wall/membrane/envelope biogenesis. We demonstrate that A. muciniphila-mediated increases in GBS aggregation and attachment to hVECs are dependent on GBS capsule and pili, respectively. Lastly, we found that A. muciniphila promoted GBS aggregation in the murine vaginal lumen and continual treatment with A. muciniphila reduced GBS vaginal persistence. Our results highlight the dynamic impact of a commensal organism on GBS colonization, as well as demonstrate the probiotic potential of A. muciniphila in the vaginal environment.

ORGANISM(S): Akkermansia muciniphila Homo sapiens Streptococcus agalactiae

PROVIDER: GSE306314 | GEO | 2026/04/15

REPOSITORIES: GEO

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