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Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage (site(+), mgm182) vs control (site(-), mgm181)

ABSTRACT: Transcriptional profiling of Mycobacterium smegmatis comparing a strain undergoing I-SceI generated DNA damage at a single genomic locus versus a control strain with no I-SceI recognition sequence in its genome (and thus not undergoing double strand DNA breaks Gene designations are from the original annotation, not the updated

ORGANISM(S): Mycolicibacterium smegmatis

PROVIDER: GSE30633 | GEO | 2011/07/15

SECONDARY ACCESSION(S): PRJNA144623

REPOSITORIES: GEO

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Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))

2011-07-15 | GSE30635 | GEO

Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage (site(+), mgm182) vs control (site(-), mgm181)

Project description:Transcriptional profiling of Mycobacterium smegmatis comparing a strain undergoing I-SceI generated DNA damage at a single genomic locus versus a control strain with no I-SceI recognition sequence in its genome (and thus not undergoing double strand DNA breaks Gene designations are from the original annotation, not the updated Five conditions investigated: Log phase cultures without induction of I-SceI expression (T=0, -Atc), Log phase cultures induced to express I-SceI by the addition of ATc for 45 minutes (T=45, +ATc), Stationary cultures control without induction of I-SceI expression (T=0, -Atc), Stationary phase cultures induced to express I-SceI by the addition of ATc for 15 minutes (T=15, +ATc), and Stationary cultures induced to express I-SceI for 45 minutes by the addition of ATc (T=45, +ATc). 3 biological replicates of each strain for each experiment.

2011-07-14 | E-GEOD-30633 | biostudies-arrayexpress

Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))

Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation Four comparisons were made, all with log phase cultures and no ATc added. Darr Site(+) with an empty vector compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing WT Arr compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing ArrH18A compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing Arr D83A compared to wild-type M. smegmatis Site(+). 3 biological replicates of each strain for each experiment.

2011-07-14 | E-GEOD-30635 | biostudies-arrayexpress

Mycolicibacterium smegmatis

Project description:Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage (site(+), mgm182) vs control (site(-), mgm181)

| PRJNA144623 | ENA

Comparison of gene expression after induction of DNA endonuclease in strains either containing a cut site or not

Project description:Investigating the induction of the SOS response after the introduction of a single dsDNA break per genome by expressing the SceI endonuclease. The endonuclease is under the control of a xylose-inducible promoter.

2008-11-21 | GSE13545 | GEO

Micobacterium smegmatis LC-MS/MS

Project description:Proteome comparison of two Mycolicibacterium smegmatis strains, mc2155 and the recombinant strain expressing MTS1338, a small non-coding RNA of Mycobacterium tuberculosis. The recombinant strain was obtained by electroporation of MTS1338-expressing plasmid into M. smegmatis mc2155 cells

2021-09-09 | PXD019813 | Pride

Mycobacterium smegmatis - Comparison of wild type SMR5 and glnR deletion strain MH1 under nitrogen starvation

Project description:In this approach, total transcript of the Mycobacterium smegmatis wild type SMR5 and a glnR deletion strain was compared under nitrogen starvation. Transcript levels of 6 genes were enhanced, 125 genes reduced in the mutant strain. This experiment confirmed GlnR as the global regulator of nitrogen metabolism in M. smegmatis. Two biological replicates were analyzed.

2011-07-01 | GSE30234 | GEO

RNA deep sequencing data of the whole transcriptomes of the wild-type and crp1 (MSMEG_6189) mutant strains of Mycobacterium smegmatis MC2 155

Project description:Our comparative RNA sequencing analysis of the wild-type strain of M. smegmatis and its isogenic crp1 (MSMEG_6189) mutant strain lacking the cAMP receptor protein 1 revealed Crp1 regulon in the M. smegmatis

2020-09-18 | GSE158137 | GEO

RNA deep sequencing data of the whole transcriptomes of the wild-type and crp2 (MSMEG_0539) mutant strains of Mycobacterium smegmatis MC2 155

Project description:Our comparative RNA sequencing analysis of the wild-type strain of M. smegmatis and its isogenic crp2 (MSMEG_0539) mutant strain lacking the cAMP receptor protein 2 revealed Crp2 regulon in the M. smegmatis

2022-05-20 | GSE203178 | GEO

RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r)

Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.

2022-12-24 | GSE221406 | GEO

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