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Perturbational single-cell RNA sequencing of 104 compounds across six immortalized and four primary cell lines

ABSTRACT: wei introduce a new perturbational scRNA-seq dataset that includes six immortalized lines (A549, A375, H1AE, HEK293T, HEP2G, and PC3) and four primary lines: CD8+ T-cells (CD8+), CD34+ hematopoietic progenitors (CD34+), primary adipocytes (PAD), and bronchial epithelial cells (HBEC). 104 compounds, including 88 from CMap, were tested across the 10 cell types in duplicate with library-matched controls, resulting in 1,737 scRNA-seq samples comprising a total of 1.26 M cells. We provide raw and demultiplexed count matrices, as well as differential expression statistics (using limma and v-scores, described below) quantifying the change in each gene relative to library-matched controls.

ORGANISM(S): Homo sapiens

PROVIDER: GSE306429 | GEO | 2025/10/23

REPOSITORIES: GEO

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