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Characterising the effect of age and sex on post-transcriptional regulation in synovial joint tissues

ABSTRACT: Our analysis determined the effect of age on polyadenylation site usage in joint tissues. Using total RNA purified from articular cartilage or synovium, we performed QuantSeq-Rev 3’ end RNA sequencing to identify polyadenylation sites utilised in all transcripts. This 3’ end sequencing method reads 3’ to 5’, starting in the polyA tail of the transcript, and so every read identifies a polyadenylation site. We then utilised PolyAminer software, with adaptations made to the code to identify and mask genomic polyA regions so that they would contribute to the site comparisons following read alignment. We found evidence that age affected polyadenylation site usage in both of these tissues

ORGANISM(S): Equus caballus

PROVIDER: GSE306645 | GEO | 2025/09/18

REPOSITORIES: GEO

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