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Single-cell RNA sequencing of Pacific white shrimp hepatopancreas infected with Vibrio parahaemolyticus

ABSTRACT: The shrimp aquaculture industry is vulnerable to large losses due to acute hepatopancreatic necrosis disease (AHPND), caused by the bacterium Vibrio parahaemolyticus. The mechanism by which the pathogen causes disease, and the host immune response, is not completely understood. The shrimp hepatopancreas is a multi-functional organ with roles in digestion, immunity, molting and reproduction. Therefore, we set out to characterize the cells of the hepatopancreas and the host response to Vibrio parahaemolyticus infection at single-cell resolution. The hepatopancreas from three individual shrimp were processed to create a single-cell transcriptomic atlas. Then the hepatopancreas from three Vibrio parahaemolyticus infected and two mocked treated shrimp were sampled for infection study. All single-cell libraries were generated using the 10X Genomics platform and sequenced on an Illumina sequencer. Data were aligned to the Litopenaeus vannamei reference genome using Cell Ranger. Seurat and clusterProfiler were used for downstream analyses. Cells of the hepatopancreas were characterized and the transcriptomic response to AHPND-causing V. parahaemolyticus was examined. Data will inform further functional studies and has the potential to aid in the development of novel preventative measures or treatments.

ORGANISM(S): Penaeus vannamei

PROVIDER: GSE306674 | GEO | 2026/02/18

REPOSITORIES: GEO

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Transcriptome analysis of pacific white shrimp (Litopenaeus vannamei) hepatopancreas in response to Vibrio parahaemolyticus inoculation

Project description:In order to gain a better understanding of the impact of Vibrio parahaemolyticus infection on genetic regulation of Litopenaeus vannamei,we performed a transcriptome analysis in the hepatopancreas of Litopenaeus vannamei challenged with Vibrio parahaemolyticus, using the Illumina HiSeq 2500 platform.

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Identification of genes associated with resistance to acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei

Project description:Acute hepatopancreatic necrosis disease (AHPND) is a shrimp farming disease, caused by a pathogenic Vibrio parahaemolyticus carrying a plasmid encoding Vp_PirAB-like toxin (VpAHPND). Whiteleg shrimp, Litopenaeus vannamei were fed food pellets containing formalin-killed VpAHPND (FKC-VpAHPND) to select for toxin resistance. To identify genes associated with Vp_PirAB-like toxin resistance, total RNA was sequenced to identify differentially expressed genes (DEGs) in the stomach and hepatopancreas among surviving shrimp (sur-FKC), AHPND-infected shrimp (Vp-inf) and normal shrimp (control). From a total of 79,591 genes, 194 and 224 DEGs were identified in the stomach and hepatopancreas transcriptomes, respectfully. The expressions of DEGs were validated by qPCR of ten genes. Only one gene, a gene homologous to L vannamei anti-lipopolysaccharide factor AV-R isoform (LvALF AV-R), was expressed significantly more strongly in sur-FKC than in the other groups. The association of LvALF AV-R expression and toxin resistance was affirmed from the surviving shrimp in a second-trial of FKC-VpAHPND feeding. These results suggest that LvALF AV-R may be involved in shrimp defense mechanisms against Vp_PirAB-like toxin virulence.

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Pacific white shrimp (Litopenaeus vannamei) hepatopancreas response to Vibrio parahaemolyticus inoculation

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Project description:In the process of shrimp farming, Vibrio parahaemolyticus carrying virulent plasmids (VpAHPND) is one of the primary pathogens causing acute hepatopancreatic necrosis disease (AHPND). Understanding the resistance mechanisms of shrimp against VpAHPND is beneficial for the development of effective treatment and breeding strategies. To systematically comprehend the resistance mechanisms, we utilized a comparative proteomic approach to analyze the different protein expression profiles in the hepatopancreas of two Litopenaeus vannamei families with different resistances to AHPND. We discovered that, without VpAHPND infection, the proteins highly expressed in the resistant family primarily participate in immune processes such as hemocyte proliferation, differentiation, apoptosis, autophagy, phagocytosis, lectins, antibacterial, and bactericidal activities; whereas most proteins involved in the digestion and metabolism of proteins, lipids, vitamins, glucose, carbohydrates, and serine metabolism were highly expressed in the susceptible family. Interestingly, after VpAHPND challenge, most of the identified immune-related proteins remained highly expressed in the resistant family, while most of the metabolism-related proteins continued to be highly expressed in the susceptible family. These findings suggest that the different expression profiles of proteins related to immunity and metabolism in the hepatopancreas may be a significant factor contributing to the varying resistances of shrimp to VpAHPND. These results offer a reference for understanding the resistance mechanisms of shrimp against VpAHPND and provide theoretical support for the development of effective breeding strategies of anti-VpAHPND shrimp varieties.

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