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Stem Cell Antigen-1 (Sca-1) Regulates Mammary Tumor Development and Cell Migration

ABSTRACT: Stem cell antigen-1 (Sca-1 or Ly6A) is a member of the Ly6 family of glycosyl phostidylinositol (GPI)-anchored cell surface proteins. To determine the potential mechanisms by which Sca-1 regulates cell migration, adhesion, and tumor development; we performed an Affymetrix mouse genome 430A 2.0 array on cDNA comparing shLuc and shSca-1 from cells grown in vitro.

ORGANISM(S): Mus musculus

PROVIDER: GSE30684 | GEO | 2012/02/16

SECONDARY ACCESSION(S): PRJNA144435

REPOSITORIES: GEO

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Stem Cell Antigen-1 (Sca-1) Regulates Mammary Tumor Development and Cell Migration

Project description:Stem cell antigen-1 (Sca-1 or Ly6A) is a member of the Ly6 family of glycosyl phostidylinositol (GPI)-anchored cell surface proteins. To determine the potential mechanisms by which Sca-1 regulates cell migration, adhesion, and tumor development; we performed an Affymetrix mouse genome 430A 2.0 array on cDNA comparing shLuc and shSca-1 from cells grown in vitro. We performed an Affymetrix mouse genome 430A 2.0 array on cDNA comparing shLuc and shSca-1 from cells grown in vitro. Experimental groups were profiled in biological triplicate.

2012-02-16 | E-GEOD-30684 | biostudies-arrayexpress

Proteomic profiling of FAC-sorted ex vivo and in vitro expanded Sca-1+cells derived from murine skeletal muscle

Project description:Stem cells antigen-1 (Sca-1) is an 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein that has widely been used for isolation of murine hematopoietic stem cells (HSCs). However, recently several studies have reported the expression of this protein in non-HSCs such as the liver, skin and muscles among others. In the current study, we used fluorescence-activated cell sorting (FACS) technique to sort Sca-1+ stem cells from mouse hindlimb muscles. The Sca-1+ cells were divided into two sets first set of Sca-1+ cells were processed for protein extraction immediately after sorting (ex vivo set) while the second set of samples, referred to as in vitro set, were expanded in cell culture after sorting and harvested after two passage and then processed for protein extraction. A high-resolution proteome map of the ex vivo and in vitro sets was generated Orbitrap Fusion Tribrid Mass Spectrometer. In total, the analysis led to the identification of 5,581 protein groups.

2021-06-21 | PXD022247 | Pride

Paired-end Sequencing of Transcriptomes of human cells expressing the human gene LY6S

Project description:We discovered a new human gene, LY6S, coding for an LY6-like protein. TransMap and phylogenetic studies reveal that LY6S is the homolog of the murine Ly6a gene known as Ly6A.2/Ly6E.1, Sca1 (Stem Cell Antigen-1) and TAP (T-cell activating protein) which codes for a cell-surface protein used extensively as a hematopoietic stem cell marker. LY6S is interferon-inducible and codes for both GPI-linked membrane and secreted proteins. The LY6S cell-surface protein, contains only 9 cysteines distinguishing it from all other LY6-like proteins that have 10 consensus cysteine residues. LY6S is most highly expressed in cells of the human spleen that constitute a unique spleen cell lineage. Expression of the GPI-linked LY6S protein in various human cell lines leads to profound changes in cell morphology and cell growth, neither of which are affected by expression of the secreted LY6S protein. RNA-Seq analyses presented here show distinct shifts in patterns of gene expression, particularly of genes coding for inflammatory and immune response proteins, were associated with these changes. Our findings reveal the presence of a previously non-annotated human gene, LY6S, which is the Ly6a homolog and that codes for proteins expressed in spleen cells representing a new cell lineage, are associated with a florid inflammatory phenotype and regulate immune responses and cell growth.

2022-01-11 | GSE159456 | GEO

Next Generation Sequencing of Transcriptomes of human cells expressing the human gene LY6S which is the TransMap and phylogeneticlly predicted homolog to murine Ly6a also known as T-cell Activating Protein, Sca1, Stem Cell Antigen-1, Ly6A2/E1 [M12 YDFR]

2022-03-15 | GSE188923 | GEO

2022-03-15 | GSE149845 | GEO

Homo sapiens

Project description:GPI-anchored Timp1 protein

| PRJNA173439 | ENA

Comparison of gene expression profiles of Sca-1+ and Sca-1- cells post alveolar injury induced by P. aeruginosa

Project description:Alveolar type II cells were isolated from mice before P. aeruginosa injection (basal state) and at 72 h post-PA, and were separated into Sca-1+ and Sca-1- populations. Gene expression profile in the following four groups of cells were compared: non-PA Sca-1-, non-PA Sca-1+, 72 h post-PA Sca-1- and 72 h post-PA Sca-1+.

2014-12-31 | GSE47600 | GEO

Anaerovirgula multivorans strain:SCA

Project description:Anaerovirgula multivorans SCA genome sequencing

| PRJNA334317 | ENA

Gene expression profile of Sca-1+ versus Sca-1- endothelial cells selected from the bone marrow of normal B6 mice

Project description:Different gene expression profile was observed in bone marrow Sca-1+ versus Sca-1- endothelial cells

2021-08-18 | GSE180104 | GEO

Apatite nanoparticle(sCA)-siTIMP1 delivery in NIH3T3

Project description:Expression profiling of sCA-siRNA delivery in NIH3T3 cells (siControl vs. siTIMP1) Goal was to determine the off target effects of sCA-siTIMP1 in NIH3T3 cells.

2020-08-27 | GSE128124 | GEO

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