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Effect of Dox-induced Cas13 expression in stable cell lines on the mouse transcriptome and co-cultured human PSCs

ABSTRACT: In the mouse EpiSCs-iCas13 stable cell line, addition of doxycycline induces Cas13 expression, which further triggers collateral effects and slows down cell proliferation. In co-culture of mouse and human cells, under normal conditions, mouse EpiSCs hold a proliferative advantage, whereas human PSCs are at a disadvantage and show almost no growth. Upon doxycycline treatment in the co-culture, Cas13 expression in mouse cells triggers collateral effects that reduce their proliferation, thereby providing a relative advantage for human PSCs. As a result, the proliferation of human PSCs is markedly enhanced, showing a growth pattern more comparable to that observed in separate cultures of human PSCs.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE307132 | GEO | 2025/12/29

REPOSITORIES: GEO

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