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Microarray Analysis of West Nile Virus infected Human Retinal Pigment Epithelium

ABSTRACT: Low-level infection is believed to play a role in the degradation of the outer blood retinal barrier, which is composed of retinal pigment epithelial (RPE) cells. By investigating immunopathogenic West nile virus (WNV) infected RPE via microarray, we sought to find key genes involved in a low-level viral infection, which are vital for normal immune responses.

ORGANISM(S): Homo sapiens

PROVIDER: GSE30719 | GEO | 2011/07/28

SECONDARY ACCESSION(S): PRJNA144383

REPOSITORIES: GEO

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Microarray Analysis of West Nile Virus infected Human Retinal Pigment Epithelium

Project description:Low-level infection is believed to play a role in the degradation of the outer blood retinal barrier, which is composed of retinal pigment epithelial (RPE) cells. By investigating immunopathogenic West nile virus (WNV) infected RPE via microarray, we sought to find key genes involved in a low-level viral infection, which are vital for normal immune responses. We infected primary human RPE cells and an human RPE cell line with WNV and extracted RNA to hybridize onto Affymetrix arrays. We were interested in the global expression of differentially regulated genes in WNV infected RPE cells, at 24h post infection. We were also interested in seeing the differences in response between infected primaries and the infected ARPE19 cell line.

2011-07-27 | E-GEOD-30719 | biostudies-arrayexpress

A Mouse Model of Chronic West Nile Virus Infection

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2012-12-29 | E-GEOD-43190 | biostudies-arrayexpress

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Project description:Purpose of this experiment was to further understand how innate immune defenses impact host response and West Nile virus tissue tropism. This study examined host-transcriptional response to West Nile virus in permissive and nonpermissive tissues using wildtype mice and mice with genetically altered interferon signaling pathways. Age-matched six to twelve week old mice were inoculated subcutaneously in the left rear footpad with 100 PFU of West Nile virus isolate TX 2002-HC (WNV-TX) in a 10 microL inoculum diluted in Hanks balanced salt solution (HBSS) supplemented with 1% heat-inactivated FBS. Mice were monitored daily for morbidity and mortality. Expression oligonucleotide arrays were performed on RNA isolated from spleen and liver tissues from strain and time-matched mock infected mice (n=2) and WNV-TX infected wild type (WT; n=3; day 4 post-infection), Ips-1-/-(n=3; day 4 post-infection), Ifnar-/- (n=3; day 2 post-infection), and Ips-1-/-xIfnar-/- (DKO; n=3; day 4 post-infection) mice.

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Project description:The purpose of this experiment was to further our understanding of gene expression in the central nervous system (thalamus and cerebrum) after exposure to West Nile virus. To that end, three different analyses were performed. The first examined differences in gene expression between horses not vaccinated and exposed to WNV and normal control horses (exposure). The second examined differences in gene expression between horses not vaccinated and exposed to WNV and horses vaccinated and exposed to WNV (survival). And the third examined differences between the nonvaccinated cerebrum and nonvaccinated thalamus of horses exposed to WNV (location). Six conditions- Gene expression in the thalamus and cerebrum of three different groups of horses (Non-vaccinated horses exposed to West Nile virus, Vaccinated horses exposed to West Nile virus, normal horses not exposed to West Nile virus). Biological replicates- 6 normal cerebrums, 6 normal thalamus, 6 vaccinated and exposed cerebrums, 6 vaccinated and exposed thalamus, 6 non-vaccinated and exposed cerebrum, 6 non-vaccinated and exposed thalamus.

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Mouse cortex transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCT001_miRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cortex infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt).

2016-03-12 | GSE77161 | GEO

Mouse cortex transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCT001_mRNA]

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Mouse cerebellum transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCB001_microRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cerebellum infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt).

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