Dataset Information

Analysis of differential expression of circular RNAs between psoriatic lesional tissues and healthy skin tissues

ABSTRACT: Psoriasis is a chronic inflammatory skin disorder characterized by abnormal keratinocyte proliferation and immune dysregulation. Circular RNAs (circRNAs) have emerged as important regulators of gene expression in various diseases, but their expression patterns and potential roles in psoriasis remain incompletely understood.In this study, we performed circular RNA sequencing on lesional skin tissues from 3 psoriasis patients and normal skin tissues from 2 healthy individuals. The identified DEcircRNAs, particularly circLDLR, may serve as promising diagnostic biomarkers or therapeutic targets for psoriasis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE307305 | GEO | 2025/09/04

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Background: Plaque psoriasis is a chronic autoimmune disorder characterized by the development of red scaly plaques. To date psoriasis lesional skin transcriptome has been extensively studied, whereas only few proteomic studies of psoriatic skin are available. Aim: The aim of this study was to compare protein expression patterns of lesional and normally looking skin of psoriasis patients with skin of the healthy volunteers, reveal differentially expressed proteins and identify changes in cell metabolism caused by the disease. Methods: Skin samples of normally looking and lesional skin donated by psoriasis patients (n = 5) and samples of healthy skin donated by volunteers (n = 5) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After protein identification and data processing, the set of differentially expressed proteins was subjected to protein ontology analysis to characterize changes in biological processes, cell components and molecular functions in the patients' skin compared to skin of the healthy volunteers. Results: The performed analysis identified 405 and 59 differentially expressed proteins in lesional and normally looking psoriatic skin compared to healthy control. We discovered decreased expression of KNG1, APOE, HRG, THBS1 and PLG in normally looking skin of the patients. Presumably, these changes were needed to protect the epidermis from spontaneous activation of kallikrein-kinin system and delay the following development of inflammatory response. In lesional skin, we identified several large groups of proteins with coordinated expression. Mainly, these proteins were involved in different aspects of protein and RNA metabolism, namely ATP synthesis and consumption; intracellular trafficking of membrane-bound vesicles, pre-RNA processing, translation, chaperoning and degradation in proteasomes/immunoproteasomes. Conclusion: Our findings explain the molecular basis of metabolic changes caused by disease in skin lesions, such as faster cell turnover and higher metabolic rate. They also indicate on downregulation of kallikrein-kinin system in normally looking skin of the patients that would be needed to delay exacerbation of the disease.

Dataset Information

Analysis of differential expression of circular RNAs between psoriatic lesional tissues and healthy skin tissues

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets