Organ-on-a-chip reveals the synergistic effect of mregDC and Treg in non-small cell lung cancer

ABSTRACT: Since the introduction of the first lung alveolus organ-on-a-chip, organ-on-a-chip technology has evolved over 15 years and is now widely applied in disease modeling, drug development, toxicological assessment, and personalized precision medicine due to its high biomimicry, dynamic and mechanical microenvironment simulation, and multi-cellular co-culture capabilities. Here, we utilized and developed a tumor metastasis organ-on-a-chip (TMOC) model to systematically investigate how modulating mregDC-Treg interactions can alleviate T cell exhaustion and tumor metastasis. To precisely study how mregDCs shape T cell exhaustion in the NSCLC TME, our TMOC spontaneously generates a hypoxia gradient from the tumor periphery to the center, mimicking the real TME, while enabling real-time observation of fluorescently labeled tumor cells metastasizing to adjacent tissue niches via a pump-driven self-circulating blood flow system. This TMOC model is suitable for the study of tumor immune microenvironment and has potential application prospects in other tumor metastasis studies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE307324 | GEO | 2025/09/11

REPOSITORIES: GEO