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Interferon stimulated gene ISG20 induces an innate immune defense signature in primary human fibroblasts [SLAM-seq]

ABSTRACT: ISG20 is an interferon-regulated protein that exhibits Rnase activity thereby inhibiting the replication of a broad spectrum of RNA viruses. We identified ISG20 as a potential restriction factor against human cytomegalovirus (HCMV) that inhibits HCMV gene expression at the early stage of infection.In order to investigate whether ISG20 affects the half-life of cellular RNAs via its putative RNase activity, a SLAMseq experiment was performed. Interestingly, ISG20 expression resulted in an overall increased stablilty of cellular RNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE307692 | GEO | 2026/01/05

REPOSITORIES: GEO

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Interferon stimulated gene ISG20 induces an innate immune defense signature in primary human fibroblasts [RNA-Seq]

Project description:ISG20 is an interferon-regulated protein that exhibits RNase activity thereby inhibiting the replication of a broad spectrum of RNA viruses. We identified ISG20 as a potential restriction factor against human cytomegalovirus (HCMV) that inhibits HCMV gene expression at the early stage of infection. However, neither the half-life of viral nor of cellular RNAs nor of viral DNA was decreased in ISG20-expressing cells. Instead, RNA-seq analysis revealed an innate immune defense signature upon ISG20 expression that comprised a distinct set of interferon-stimulated genes, zinc finger proteins and transposable elements.

2026-01-05 | GSE307691 | GEO

Interferon stimulated gene ISG20 induces an innate immune defense signature in primary human fibroblasts

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Interferon stimulated gene ISG20 induces an innate immune defense signature in primary human fibroblasts [SLAM-seq]

Project description:Interferon stimulated gene ISG20 induces an innate immune defense signature in primary human fibroblasts [SLAM-seq]

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Analysis of Roquin targetome during HCMV infection

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