Assay for transposase-accessible chromatin using sequencing (ATAC-Seq) of Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria hordei (Bh); isolate 5874)

ABSTRACT: Transcriptional reprogramming is a key component of disease resistance. MLA (mildew locus a), is an immune receptor that interacts with several transcription factors (TFs), and thus, represents a model to explore regulatory architecture during defense. To characterize chromatin accessibility of barley during powdery mildew infection, a series of isogenic mutant genotypes and timepoints were subjected to ATAC sequencing. We designed our study around two diverse genes in host immunity, mildew locus a (Mla6), a nucleotide binding leucine-rich repeat (NLR) immune receptor, and the Blufensin1 (Bln1) basal defense regulator, which influence immunity in a resistance (R)-gene dependent and R-gene independent manner, respectively. In addition to the CI 16151 resistant progenitor (Mla6, Bln1), three mutants were utilized: the susceptible mla6-m18982, the resistant bln1-m19089, and the susceptible double mutant (mla6+bln1)-m19028 (Chapman et al., 2021; Velásquez-Zapata et al., 2024). Four infection timepoints (0, 16, 20, 32 HAI) were taken to sample across key pathogen life cycle events such as appressorial penetration and haustorial development. Validation of the ATAC-Seq dataset was performed using the correlation with gene expression profiles from RNA-Seq data; by checking the presence of epistatic events at the chromatin level, phenomenon that was recently described using RNA-Seq (Velásquez-Zapata et al., 2024).

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE307853 | GEO | 2026/03/26

REPOSITORIES: GEO