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Expression profile of MRP14(S100A9)-positive and -negative mouse bone marrow granulocytes under different stimulations

ABSTRACT: MRP14 is tissue-specific expressed only in granulocytes, monocytes and in inflammation-activated keratinocytes. Its existence in the early phase of inflammation may be a hint towards a participation in immune response. Intra- and extracellular functions have been described yet. An antibacterial effect is caused by chelation of zinc, which then cannot be used for bacterial growth. The experiments were performed to shed light on the function of the SA100 protein MRP14. Keywords: cell type comparison, response to stimulus

ORGANISM(S): Mus musculus

PROVIDER: GSE3079 | GEO | 2006/08/08

SECONDARY ACCESSION(S): PRJNA93083

REPOSITORIES: GEO

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Transcription profiling of mouse MRP14(S100A9)-positive and -negative bone marrow granulocytes under different stimulations

Project description:MRP14 is tissue-specific expressed only in granulocytes, monocytes and in inflammation-activated keratinocytes. Its existence in the early phase of inflammation may be a hint towards a participation in immune response. Intra- and extracellular functions have been described yet. An antibacterial effect is caused by chelation of zinc, which then cannot be used for bacterial growth. The experiments were performed to shed light on the function of the SA100 protein MRP14. Experiment Overall Design: MRP14(SA100A9)-positive and -negative mouse bone marrow granulocytes are measured unstimulated as control (2 repeats each at time point 0h, 5 repeats each at time point 4h) and then stimulated for 4h with LTB-4 (1 repeat each), A23187 (a calcium ionophore, 4 repeats each), LPS (part of gram negative bacteria, 3 repeats each) and PMA (1 repeat each); MRP14(SA100A9)-positive and -negative mouse spleen tissue (1 repeat each) for comparison; another data set contains all experiments of this data set with Mu11KsubA instead of Mu11KsubB arrays

2008-06-12 | E-GEOD-3079 | biostudies-arrayexpress

AKIRIN1 is a suitable reference gene in natural killer cells and granulocytes from patients with SIRS and septic shock [granulocytes]

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Project description:Neutrophil granulocytes are the major cells involved in the Chlamydia trachomatis (C.trachomatis)-mediated inflammation and histopathology. A key gene in human intracellular antichlamydial defense is the tryptophan degrading enzyme indoleamine 2,3-dioxygenase (IDO), which limits the growth of the tryptophan auxotroph Chlamydia. Despite its importance, the role of IDO in the intracellular defense against Chlamydia in neutrophils has not yet been characterized. Affymetrix microarrays were used to obtain global gene expression data for monitoring the effect of C. trachomatis serovar D infection on the transcriptome of human neutrophil granulocytes.

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Changes in gene expression of granulocytes during in vivo G-CSF/dexamethasone mobilization.

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Changes in gene expression of granulocytes during in vivo G-CSF/dexamethasone mobilization.

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2019-02-01 | PXD010701 | Pride

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2014-05-14 | E-GEOD-57622 | biostudies-arrayexpress

Gene expression data from mouse hemopoietic stem cells (LSKs), promyelocytes, myelocytes and granulocytes.

2014-05-14 | GSE57622 | GEO

Alarmins MRP8 and MRP14 induce stress-tolerance in phagocytes under sterile inflammatory conditions

Project description:Hyporesponsiveness by phagocytes, a well-known phenomenon in sepsis, is frequently induced by low-dose endotoxin-stimulation of Toll-like-receptor-4 (TLR4) but can also be found under sterile inflammatory conditions. We now demonstrate that the endogenous alarmins myeloid-related protein (MRP) 8 and MRP14 induce phagocyte hyporesponsiveness via chromatin modifications in a TLR4-dependent manner resulting in enhanced survival during murine septic shock. Also during sterile inflammation, polytrauma and burn patients present with initially high MRP serum concentrations identifying these proteins as obvious candidates for triggering secondary hyporesponsiveness in these patients. Interestingly, increased peripartal MRP concentrations prime human neonatal phagocytes for hyporesponsiveness, which was confirmed in murine neonatal endotoxinemia in wildtype and MRP14 -/- mice. Using a comparative bioinformatics analysis between genome-wide response patterns of MRP- and LPS- tolerized monocytes we demonstrated no difference in global gene expression between samples pretreated with either MRP8-MRP14 or LPS. Our data indicate that alarmin-triggered phagocyte tolerance represents a novel regulatory mechanism for the susceptibility of neonates to systemic infections and during sterile inflammation. Human blood monocytes prestimulated with MRP8-MRP14 or LPS and afterwards activated with LPS were selected for RNA extraction and hybridization on Illumina microarrays.

2014-12-12 | E-GEOD-61477 | biostudies-arrayexpress

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