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Extraction of high quality total RNA from liquid nitrogen stocks

ABSTRACT: Microarray expression arrays on THP-1 acute myeloid leukemia cell line samples, contrasting expression profiles of freshly harvested cell pellets, cells replated into fresh media for 24 hours and cells directly pelleted from liquid nitrogen stocks.

ORGANISM(S): Homo sapiens

PROVIDER: GSE30819 | GEO | 2012/12/25

SECONDARY ACCESSION(S): PRJNA144201

REPOSITORIES: GEO

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Extraction of high quality total RNA from liquid nitrogen stocks

Project description:Microarray expression arrays on THP-1 acute myeloid leukemia cell line samples, contrasting expression profiles of freshly harvested cell pellets, cells replated into fresh media for 24 hours and cells directly pelleted from liquid nitrogen stocks. Nine samples were analysed, composed of 3 biological replicates for each state (fresh, replated, frozen)

2012-12-25 | E-GEOD-30819 | biostudies-arrayexpress

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