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ChIP-seq analysis of IRF1 binding in VSV-infected bone marrow-derived macrophages (BMDMs)

ABSTRACT: This study examines the genome-wide binding profile of the transcription factor IRF1 in bone marrow-derived macrophages (BMDMs) infected with vesicular stomatitis virus (VSV) compared to uninfected controls. BMDMs were differentiated from mouse bone marrow cells via M-CSF stimulation and then infected with VSV (MOI = 1) for 4 hours or mock-treated. Chromatin immunoprecipitation (ChIP) was performed using an anti-IRF1 antibody, followed by next-generation sequencing (Illumina). Data analysis included alignment to the mm10 genome, peak calling, and coverage normalization to identify IRF1 binding sites under viral infection. These results provide insights into IRF1's regulatory role in antiviral immune responses.

ORGANISM(S): Mus musculus

PROVIDER: GSE308320 | GEO | 2026/03/31

REPOSITORIES: GEO

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