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Rapid production of precisely edited cattle and mice via a single AAV delivered miniature IscB#/ωRNA# [dataset 2]

ABSTRACT: Utilising adeno-associated virus (AAV) to deliver CRISPR/Cas has enhanced the generation of knock-out mice, but single-AAV delivery of Cas and homology-directed-repair (HDR) templates in livestock remains a challenge. Here, we introduce all-in-one AAV vectors containing shortened IscB#, the progenitor of Cas9, along with either miniature ωRNA#, or ωRNA# with a large HDR-template. Encouragingly, we efficiently generated knock-out and knock-in cattle and mice by infecting zygotes with AAV::IscB#/ωRNA#-HDR without costly micromanipulators.

ORGANISM(S): Homo sapiens

PROVIDER: GSE308344 | GEO | 2026/01/19

REPOSITORIES: GEO

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Rapid production of precisely edited cattle and mice via a single AAV delivered miniature IscB#/ωRNA# [dataset 1]

Project description:Utilising adeno-associated virus (AAV) to deliver CRISPR/Cas has enhanced the generation of knock-out mice, but single-AAV delivery of Cas and homology-directed-repair (HDR) templates in livestock remains a challenge. Here, we introduce all-in-one AAV vectors containing shortened IscB#, the progenitor of Cas9, along with either miniature ωRNA#, or ωRNA# with a large HDR-template. Encouragingly, we efficiently generated knock-out and knock-in cattle and mice by infecting zygotes with AAV::IscB#/ωRNA#-HDR without costly micromanipulators.

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Rapid production of precisely edited cattle and mice via a single AAV delivered miniature IscB#/ωRNA# [dataset 2]

Project description:Rapid production of precisely edited cattle and mice via a single AAV delivered miniature IscB#/ωRNA# [dataset 2]

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Rapid production of precisely edited cattle and mice via a single AAV delivered miniature IscB#/ωRNA# [dataset 1]

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