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Transcriptomic analysis of ALPK1-dependent responses in BMDMs from WT and ALPK1 knockout C57BL/6 mice upon ADP-Hep stimulation

ABSTRACT: The ALPK1 pathway is a cytosolic sensor for the bacterial metabolite ADP-heptose (ADP-Hep), triggering innate immune signalling. To investigate ALPK1-dependent transcriptional responses, bone marrow-derived macrophages (BMDMs) were generated from wild-type (WT) and Alpk1 knockout C57BL/6 mice using L929 cell-conditioned medium. On day 7 of differentiation, BMDMs were treated with either PBS (control) or ADP-Hep. RNA sequencing was performed to characterise gene expression changes, allowing assessment of ALPK1 pathway activation.

ORGANISM(S): Mus musculus

PROVIDER: GSE308482 | GEO | 2025/10/10

REPOSITORIES: GEO

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