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Effect of MDM2 overexpression on response to etoposide treatment

ABSTRACT: We aimed to study the effect of non-mutational p53 inactivation on the response of hematopoietic progenitor cells to genotoxic treatment. In order to study this in vitro, we generated HSPC lines by enforced ER-Hoxb8 expression in bone marrow cells derived from transgenic Trp53-fl-R245W-GFP mice. In the absence of Cre-mediated recombination, these cells are wild-type for Trp53. We additionally transduced these cells lentivirally with a construct overexpressing murine Mdm2 or a control vector. This leads to non-mutational inactivation of functional p53. Thereby, we aimed to compare the transcriptional response to genotoxic treatment in Trp53 wild-type cells with or without non-mutational p53 inactivation through Mdm2 overexpression

ORGANISM(S): Mus musculus

PROVIDER: GSE308508 | GEO | 2025/09/23

REPOSITORIES: GEO

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The effect of MDM2 overexpression on the cellular response to etoposide treatment

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