Cancer cells transfer invasive properties through microRNAs contained in collagen-tracks
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ABSTRACT: Invasion is a prerequisite for metastasis formation. During tumor development, type I collagen overexpression increases tumor microenvironment stiffness, facilitating cancer cell dissemination. During breast cancer cell migration in 2D and 3D matrices, we observed membrane debris left behind, attached to the collagen fibrils, along the migration path. We named these structures collagen-tracks. Their formation is stimulated by the interaction between the extracellular matrix (ECM) and matrix receptors, such as the discoidin-domain receptor 1 (DDR1). They present a specific nucleic acid and protein contents. When deposited by highly invasive breast cancer cells, internalized collagen-tracks reprogram non-invasive cells into highly pro-metastatic ones by inducing a partial epithelial–mesenchymal transition (EMT) associated with an increase in ECM degradation and cell invasiveness. This cell reprogramming is dependent on specific miRNAs identified in the collagen-tracks. Collagen-tracks thus represent a new form of cell-cell communication important for driving tumor invasion that could be targeted to prevent metastasis.
ORGANISM(S): Homo sapiens
PROVIDER: GSE308896 | GEO | 2025/12/14
REPOSITORIES: GEO
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