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Transcriptional profiling of tumor infiltrating neutrophils in a mouse model (MOC1) of oral cavity cancer

ABSTRACT: Neutrophils are abundant in the tumor microenvironment and frequently acquire immunosuppressive phenotypes, yet the mechanisms driving this transition remain incompletely defined. To investigate the role of the transcription factor Egr1 and MEK signaling in neutrophil-mediated immune suppression, we performed single-cell RNA sequencing (scRNA-seq) of tumor-infiltrating neutrophils isolated from a syngeneic oral cavity cancer model (MOC1) in mice. This dataset comprises four experimental groups: Egr1 wild-type (WT) vs. Egr1 knockout (KO) and vehicle control vs. trametinib (MEK inhibitor) treatment. Comparative transcriptional profiling revealed that Egr1-deficient neutrophils fail to upregulate key immunosuppressive and pro-inflammatory effectors, including Osm, Ptgs2, and Il1b, in response to the tumor microenvironment. Conversely, trametinib-treated neutrophils displayed reduced expression of hypoxia- and MEK-driven transcriptional programs, including suppression of Osm, Ptgs2, Il1b, and the AP-1 transcription factor Junb.

ORGANISM(S): Mus musculus

PROVIDER: GSE309017 | GEO | 2025/09/25

REPOSITORIES: GEO

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